Abstract
Genetic bottlenecks are stochastic events that narrow variation in a population. We compared bottlenecks during the systemic infection of Cucumber mosaic virus (CMV) in four host plants. We mechanically inoculated an artificial population of twelve CMV mutants to young leaves of tomato, pepper, Nicotiana benthamiana, and squash. The inoculated leaves and primary and secondary systemically infected leaves were sampled at 2, 10, and 15 days post-inoculation. All twelve mutants were detected in all of the inoculated leaves. The number of mutants recovered from the systemically infected leaves of all host species was reduced significantly, indicating bottlenecks in systemic movement. The recovery frequencies of a few of the mutants were significantly different in each host probably due to host-specific selective forces. These results have implications for the differences in virus population variation that is seen in different host plants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 279-283 |
| Number of pages | 5 |
| Journal | Virology |
| Volume | 404 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 1 2010 |
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- Virology
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Genetic bottlenecks during systemic movement of Cucumber mosaic virus vary in different host plants. / Ali, Akhtar; Roossinck, Marilyn J.
In: Virology, Vol. 404, No. 2, 01.09.2010, p. 279-283.Research output: Contribution to journal › Article
TY - JOUR
T1 - Genetic bottlenecks during systemic movement of Cucumber mosaic virus vary in different host plants
AU - Ali, Akhtar
AU - Roossinck, Marilyn J.
PY - 2010/9/1
Y1 - 2010/9/1
N2 - Genetic bottlenecks are stochastic events that narrow variation in a population. We compared bottlenecks during the systemic infection of Cucumber mosaic virus (CMV) in four host plants. We mechanically inoculated an artificial population of twelve CMV mutants to young leaves of tomato, pepper, Nicotiana benthamiana, and squash. The inoculated leaves and primary and secondary systemically infected leaves were sampled at 2, 10, and 15 days post-inoculation. All twelve mutants were detected in all of the inoculated leaves. The number of mutants recovered from the systemically infected leaves of all host species was reduced significantly, indicating bottlenecks in systemic movement. The recovery frequencies of a few of the mutants were significantly different in each host probably due to host-specific selective forces. These results have implications for the differences in virus population variation that is seen in different host plants.
AB - Genetic bottlenecks are stochastic events that narrow variation in a population. We compared bottlenecks during the systemic infection of Cucumber mosaic virus (CMV) in four host plants. We mechanically inoculated an artificial population of twelve CMV mutants to young leaves of tomato, pepper, Nicotiana benthamiana, and squash. The inoculated leaves and primary and secondary systemically infected leaves were sampled at 2, 10, and 15 days post-inoculation. All twelve mutants were detected in all of the inoculated leaves. The number of mutants recovered from the systemically infected leaves of all host species was reduced significantly, indicating bottlenecks in systemic movement. The recovery frequencies of a few of the mutants were significantly different in each host probably due to host-specific selective forces. These results have implications for the differences in virus population variation that is seen in different host plants.
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U2 - 10.1016/j.virol.2010.05.017
DO - 10.1016/j.virol.2010.05.017
M3 - Article
VL - 404
SP - 279
EP - 283
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -