Genetic bottlenecks reduce population variation in an experimental RNA virus population

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Genetic bottlenecks are stochastic events that limit genetic variation in a population and result in founding populations that can lead to genetic drift. Evidence of past genetic bottlenecks in numerous biological systems, from mammals to viruses, has been described. In this study, we used an artificial population of Cucumber mosaic virus consisting of 12 restriction enzyme marker-bearing mutants. This population was inoculated onto young leaves of tobacco plants and monitored throughout the course of systemic infection. We show here that the genetic variation in a defined population of an RNA virus is significantly, stochastically, and reproducibly reduced during the systemic infection process, providing clear evidence of a genetic bottleneck.

Original languageEnglish (US)
Pages (from-to)10582-10587
Number of pages6
JournalJournal of virology
Volume78
Issue number19
DOIs
StatePublished - Oct 1 2004

Fingerprint

RNA Viruses
Population
Cucumovirus
Genetic Drift
genetic variation
Plant Leaves
Cucumber mosaic virus
genetic drift
Infection
infection
Tobacco
Mammals
tobacco
RNA viruses
mammals
Viruses
viruses
mutants
Enzymes
enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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Genetic bottlenecks reduce population variation in an experimental RNA virus population. / Li, Hongye; Roossinck, Marilyn J.

In: Journal of virology, Vol. 78, No. 19, 01.10.2004, p. 10582-10587.

Research output: Contribution to journalArticle

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