Genetic control of the immune response in mice to Leishmania mexicana surface protease

José Alejandro López, Hans Albert Reins, Robert J. Etges, Linda L. Button, W. Robert McMaster, Peter Overath, Jan Klein

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Abstract

Congenic mouse strains were tested in the lymphocyte proliferation assay for their response to the purified surface protease of Leishmania mexicana (gp63). The data obtained allow us to distinguish three different patterns of response, influenced both by H-2 (class II) and non-H-2 genes. Mice of the C57BL/10 (B10) background carrying H-2 haplotypes b,q, and r were found to be high responders; those carrying H-2 haplotypes d, j, v, and z were low responders; and those with H-2a, H-2f, H-2k, H-2p, and H-2u haplotypes were intermediate responders. Studies with H-2 recombinant strains indicated that the high responsiveness on the B10 background was determined by the Ab allele and the low responsiveness influenced by the Ad allele. Other genes besides H-2 appear to have a role in the immune response as shown by the fact that some strains with BALB, DBA, or C3H background differed in their pattern of responsiveness from BlO background strains carrying the corresponding H-2 haplotypes. By using recombinant protein, the influence of the leishmanial surface lipophosphoglycan that might co-purify with gp63, on the MHC restriction of the response to gp63 was excluded. The immune response to gp63 did not correlate with susceptibility of mouse strains to cutaneous infection with L. mexicana promastigotes.

Original languageEnglish (US)
Pages (from-to)1328-1334
Number of pages7
JournalJournal of Immunology
Volume146
Issue number4
Publication statusPublished - Feb 15 1991

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

López, J. A., Reins, H. A., Etges, R. J., Button, L. L., McMaster, W. R., Overath, P., & Klein, J. (1991). Genetic control of the immune response in mice to Leishmania mexicana surface protease. Journal of Immunology, 146(4), 1328-1334.