Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children

C. A. Contreras, T. J. Ochoa, J. Ruiz, D. W. Lacher, D. Durand, C. Debroy, C. F. Lanata, T. G. Cleary

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (P<0.001); 28.9% of diarrhoeal and 17.6% of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P<0.05). espD-alpha was more common among acute episodes (P<0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.

Original languageEnglish (US)
Pages (from-to)1114-1120
Number of pages7
JournalJournal of Medical Microbiology
Volume61
Issue number8
DOIs
StatePublished - Aug 1 2012

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Enteropathogenic Escherichia coli
Genetic Loci
Enterocytes
Genes
Alleles
Gene Frequency
Restriction Fragment Length Polymorphisms
Diarrhea
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Contreras, C. A. ; Ochoa, T. J. ; Ruiz, J. ; Lacher, D. W. ; Durand, D. ; Debroy, C. ; Lanata, C. F. ; Cleary, T. G. / Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children. In: Journal of Medical Microbiology. 2012 ; Vol. 61, No. 8. pp. 1114-1120.
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abstract = "The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 {\%}) than from diarrhoeal samples (90/936, 9.6 {\%}) (P<0.001); 28.9{\%} of diarrhoeal and 17.6{\%} of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P<0.05). espD-alpha was more common among acute episodes (P<0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.",
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Contreras, CA, Ochoa, TJ, Ruiz, J, Lacher, DW, Durand, D, Debroy, C, Lanata, CF & Cleary, TG 2012, 'Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children', Journal of Medical Microbiology, vol. 61, no. 8, pp. 1114-1120. https://doi.org/10.1099/jmm.0.045443-0

Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children. / Contreras, C. A.; Ochoa, T. J.; Ruiz, J.; Lacher, D. W.; Durand, D.; Debroy, C.; Lanata, C. F.; Cleary, T. G.

In: Journal of Medical Microbiology, Vol. 61, No. 8, 01.08.2012, p. 1114-1120.

Research output: Contribution to journalArticle

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T1 - Genetic diversity of locus of enterocyte effacement genes of enteropathogenic Escherichia coli isolated from Peruvian children

AU - Contreras, C. A.

AU - Ochoa, T. J.

AU - Ruiz, J.

AU - Lacher, D. W.

AU - Durand, D.

AU - Debroy, C.

AU - Lanata, C. F.

AU - Cleary, T. G.

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N2 - The aim of this study was to determine the frequency and allele associations of locus of enterocyte effacement encoded esp and tir genes among 181 enteropathogenic Escherichia coli (EPEC) strains (90 diarrhoea-associated and 91 controls) isolated from Peruvian children under 18 months of age. We analysed espA, espB, espD and tir alleles by PCR-RFLP. EPEC strains were isolated with higher frequency from healthy controls (91/424, 21.7 %) than from diarrhoeal samples (90/936, 9.6 %) (P<0.001); 28.9% of diarrhoeal and 17.6% of control samples were typical EPEC (tEPEC). The distribution of espA alleles (alpha, beta, beta2 and gamma) and espD alleles (alpha, beta, gamma and a new variant, espD-N1) between tEPEC and atypical EPEC (aEPEC) was significantly different (P<0.05). espD-alpha was more common among acute episodes (P<0.05). espB typing resulted in five alleles (alpha, beta, gamma and two new suballeles, espB-alpha2 and espB-alpha3), while tir-beta and tir-gamma2 were the most common intimin receptor subtypes. Seventy-two combinations of espA, espB, espD and tir alleles were found; the most prevalent combination was espA-beta, espB-beta, espD-beta, tir-beta (34/181 strains), which was more frequent among tEPEC strains (P,0.05). Our findings indicate that there is a high degree of heterogeneity among EPEC strains isolated from Peruvian children and that aEPEC and tEPEC variants cluster.

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