Genetic diversity of the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins

Chaturong Putaporntip, Rachanee Udomsangpetch, Urassaya Pattanawong, Liwang Cui, Somchai Jongwutiwes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Plasmodium vivax merozoite surface protein-5 (PvMsp-5), a potential vaccine candidate, is encoded by a two-exon single copy gene. We have conducted a comprehensive analysis of PvMsp-5 by sequencing the entire gene of four parasite populations from northwestern Thailand (n=73), southern Thailand (n=53), Indonesia (n=25) and Brazil (n=24), and five isolates from other endemic areas. Results reveal that exon I exhibits a significantly higher level of nucleotide diversity at both synonymous and nonsynonymous sites than exon II (p<0.01). Neutrality tests based on both intraspecific and interspecific nucleotide polymorphism have detected a signature of positive selection in exon I of all populations while substitutions in exon II mainly followed neutral expectation except that three residues in exon II of northwestern Thailand population appear to be positively selected using the Bayes Empirical Bayes method. Short imperfect repeats were identified in exon I at an equivalent region to its orthologue in P. knowlesi, supporting their close genetic relatedness. Significant levels of population subdivision were detected among most populations including those between northwestern and southern Thailand (p<10-5), implying absent or minimal gene flow between these populations. Importantly, evidences for intragenic recombination in PvMsp-5 were found in most populations except that from southern Thailand in which haplotype diversity and nucleotide diversity were exceptionally low. Results from Fu and Li's D, F and D and F tests suggested that PvMsp-5 of most P. vivax populations have been maintained by balancing selection whereas southern Thailand population could have gone through recent bottleneck events. These findings are concordant with a substantial reduction in the number of P. vivax cases in southern Thailand during the past decade, followed by a very recent population expansion. Therefore, spatio-temporal monitoring of parasite population genetics provides important implications for disease control.

Original languageEnglish (US)
Pages (from-to)24-35
Number of pages12
JournalGene
Volume456
Issue number1-2
DOIs
StatePublished - May 1 2010

Fingerprint

Plasmodium vivax
Thailand
Exons
Population
Nucleotides
Parasites
merozoite surface protein 5
Indonesia
Gene Flow
Protein Sequence Analysis
Population Genetics
Haplotypes
Genetic Recombination
Genes
Brazil
Vaccines

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Putaporntip, C., Udomsangpetch, R., Pattanawong, U., Cui, L., & Jongwutiwes, S. (2010). Genetic diversity of the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins. Gene, 456(1-2), 24-35. https://doi.org/10.1016/j.gene.2010.02.007
Putaporntip, Chaturong ; Udomsangpetch, Rachanee ; Pattanawong, Urassaya ; Cui, Liwang ; Jongwutiwes, Somchai. / Genetic diversity of the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins. In: Gene. 2010 ; Vol. 456, No. 1-2. pp. 24-35.
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Putaporntip, C, Udomsangpetch, R, Pattanawong, U, Cui, L & Jongwutiwes, S 2010, 'Genetic diversity of the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins', Gene, vol. 456, no. 1-2, pp. 24-35. https://doi.org/10.1016/j.gene.2010.02.007

Genetic diversity of the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins. / Putaporntip, Chaturong; Udomsangpetch, Rachanee; Pattanawong, Urassaya; Cui, Liwang; Jongwutiwes, Somchai.

In: Gene, Vol. 456, No. 1-2, 01.05.2010, p. 24-35.

Research output: Contribution to journalArticle

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T1 - Genetic diversity of the Plasmodium vivax merozoite surface protein-5 locus from diverse geographic origins

AU - Putaporntip, Chaturong

AU - Udomsangpetch, Rachanee

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