Genetic diversity of the Pvk12 gene in Plasmodium vivax from the China-Myanmar border area

Shuang Deng, Yonghua Ruan, Yao Bai, Yue Hu, Zeshuai Deng, Yongshu He, Rui Ruan, Yanrui Wu, Zhaoqing Yang, Liwang Cui

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Plasmodium falciparum resistance to artemisinin emerged in the Greater Mekong Sub-region has been associated with mutations in the propeller domain of the kelch gene Pfk13. Methods: Here the polymorphisms in Pvk12 gene, the orthologue of Pfk13 in Plasmodium vivax, were determined by PCR and sequencing in 262 clinical isolates collected in recent years (2012-2015) from the China-Myanmar border area. Results: Sequencing of full-length Pvk12 genes from these isolates identified three synonymous mutations (N172N, S360S, S697S) and one non-synonymous mutation M124I, all of which were at very low prevalence (2.0-3.1%). Moreover, these mutations were non-overlapping between the two study sites on both sides of the border. Molecular evolutionary analysis detected signature of purifying selection on Pvk12. Conclusions: There is no direct evidence that Pvk12 is involved in artemisinin resistance in P. vivax, but it remains a potential candidate requiring further investigation. Continuous monitoring of potential drug resistance in this parasite is needed in order to facilitate the regional malaria elimination campaign.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalMalaria journal
Volume15
Issue number1
DOIs
StatePublished - Nov 4 2016

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Myanmar
Plasmodium vivax
China
Mutation
Genes
Plasmodium falciparum
Drug Resistance
Malaria
Parasites
Polymerase Chain Reaction
artemisinine

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Deng, Shuang ; Ruan, Yonghua ; Bai, Yao ; Hu, Yue ; Deng, Zeshuai ; He, Yongshu ; Ruan, Rui ; Wu, Yanrui ; Yang, Zhaoqing ; Cui, Liwang. / Genetic diversity of the Pvk12 gene in Plasmodium vivax from the China-Myanmar border area. In: Malaria journal. 2016 ; Vol. 15, No. 1. pp. 1-6.
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abstract = "Background: Plasmodium falciparum resistance to artemisinin emerged in the Greater Mekong Sub-region has been associated with mutations in the propeller domain of the kelch gene Pfk13. Methods: Here the polymorphisms in Pvk12 gene, the orthologue of Pfk13 in Plasmodium vivax, were determined by PCR and sequencing in 262 clinical isolates collected in recent years (2012-2015) from the China-Myanmar border area. Results: Sequencing of full-length Pvk12 genes from these isolates identified three synonymous mutations (N172N, S360S, S697S) and one non-synonymous mutation M124I, all of which were at very low prevalence (2.0-3.1{\%}). Moreover, these mutations were non-overlapping between the two study sites on both sides of the border. Molecular evolutionary analysis detected signature of purifying selection on Pvk12. Conclusions: There is no direct evidence that Pvk12 is involved in artemisinin resistance in P. vivax, but it remains a potential candidate requiring further investigation. Continuous monitoring of potential drug resistance in this parasite is needed in order to facilitate the regional malaria elimination campaign.",
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Deng, S, Ruan, Y, Bai, Y, Hu, Y, Deng, Z, He, Y, Ruan, R, Wu, Y, Yang, Z & Cui, L 2016, 'Genetic diversity of the Pvk12 gene in Plasmodium vivax from the China-Myanmar border area', Malaria journal, vol. 15, no. 1, pp. 1-6. https://doi.org/10.1186/s12936-016-1592-z

Genetic diversity of the Pvk12 gene in Plasmodium vivax from the China-Myanmar border area. / Deng, Shuang; Ruan, Yonghua; Bai, Yao; Hu, Yue; Deng, Zeshuai; He, Yongshu; Ruan, Rui; Wu, Yanrui; Yang, Zhaoqing; Cui, Liwang.

In: Malaria journal, Vol. 15, No. 1, 04.11.2016, p. 1-6.

Research output: Contribution to journalArticle

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T1 - Genetic diversity of the Pvk12 gene in Plasmodium vivax from the China-Myanmar border area

AU - Deng, Shuang

AU - Ruan, Yonghua

AU - Bai, Yao

AU - Hu, Yue

AU - Deng, Zeshuai

AU - He, Yongshu

AU - Ruan, Rui

AU - Wu, Yanrui

AU - Yang, Zhaoqing

AU - Cui, Liwang

PY - 2016/11/4

Y1 - 2016/11/4

N2 - Background: Plasmodium falciparum resistance to artemisinin emerged in the Greater Mekong Sub-region has been associated with mutations in the propeller domain of the kelch gene Pfk13. Methods: Here the polymorphisms in Pvk12 gene, the orthologue of Pfk13 in Plasmodium vivax, were determined by PCR and sequencing in 262 clinical isolates collected in recent years (2012-2015) from the China-Myanmar border area. Results: Sequencing of full-length Pvk12 genes from these isolates identified three synonymous mutations (N172N, S360S, S697S) and one non-synonymous mutation M124I, all of which were at very low prevalence (2.0-3.1%). Moreover, these mutations were non-overlapping between the two study sites on both sides of the border. Molecular evolutionary analysis detected signature of purifying selection on Pvk12. Conclusions: There is no direct evidence that Pvk12 is involved in artemisinin resistance in P. vivax, but it remains a potential candidate requiring further investigation. Continuous monitoring of potential drug resistance in this parasite is needed in order to facilitate the regional malaria elimination campaign.

AB - Background: Plasmodium falciparum resistance to artemisinin emerged in the Greater Mekong Sub-region has been associated with mutations in the propeller domain of the kelch gene Pfk13. Methods: Here the polymorphisms in Pvk12 gene, the orthologue of Pfk13 in Plasmodium vivax, were determined by PCR and sequencing in 262 clinical isolates collected in recent years (2012-2015) from the China-Myanmar border area. Results: Sequencing of full-length Pvk12 genes from these isolates identified three synonymous mutations (N172N, S360S, S697S) and one non-synonymous mutation M124I, all of which were at very low prevalence (2.0-3.1%). Moreover, these mutations were non-overlapping between the two study sites on both sides of the border. Molecular evolutionary analysis detected signature of purifying selection on Pvk12. Conclusions: There is no direct evidence that Pvk12 is involved in artemisinin resistance in P. vivax, but it remains a potential candidate requiring further investigation. Continuous monitoring of potential drug resistance in this parasite is needed in order to facilitate the regional malaria elimination campaign.

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