Genetic loci controlling lethal cell death in tomato caused by viral satellite RNA infection

Ping Xu, Hua Wang, Frank Coker, Jun Y.Ing Ma, Yuhong Tang, Mark Taylor, Marilyn J. Roossinck

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cucumber mosaic virus (CMV) associated with D satellite RNA (satRNA) causes lethal systemic necrosis (LSN) in tomato (Solanum lycopersicum), which involves programmed cell death. No resistance to this disease has been found in tomato. We obtained a line of wild tomato, S. habrochaitis, with a homogeneous non-lethal response (NLR) to the infection. This line of S. habrochaitis was crossed with tomato to generate F1 plants that survived the infection with NLR, indicating that NLR is a dominant trait. The NLR trait was successfully passed on to the next generation. The phenotype and genotype segregation was analyzed in the first backcross population. The analyses indicate that the NLR trait is determined by quantitative trait loci (QTL). Major QTL associated with the NLR trait were mapped to chromosomes 5 and 12. Results from Northern blot and in situ hybridization analyses revealed that the F1 and S. habrochaitis plants accumulated minus-strand satRNA more slowly than tomato, and fewer vascular cells were infected. In addition, D satRNA-induced LSN in tomato is correlated with higher accumulation of the minus-strand satRNA compared with the accumulation of the minus strand of a non-necrogenic mutant D satRNA.

Original languageEnglish (US)
Pages (from-to)1034-1044
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume25
Issue number8
DOIs
StatePublished - Aug 1 2012

Fingerprint

Satellite RNAs
satellite RNA
Genetic Loci
Viral RNA
Lycopersicon esculentum
lethal genes
cell death
Cell Death
tomatoes
loci
Infection
infection
Quantitative Trait Loci
quantitative trait loci
necrosis
Necrosis
Cucumovirus
Cucumber mosaic virus
Solanum lycopersicum
Chromosomes, Human, Pair 12

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

Xu, Ping ; Wang, Hua ; Coker, Frank ; Ma, Jun Y.Ing ; Tang, Yuhong ; Taylor, Mark ; Roossinck, Marilyn J. / Genetic loci controlling lethal cell death in tomato caused by viral satellite RNA infection. In: Molecular Plant-Microbe Interactions. 2012 ; Vol. 25, No. 8. pp. 1034-1044.
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Genetic loci controlling lethal cell death in tomato caused by viral satellite RNA infection. / Xu, Ping; Wang, Hua; Coker, Frank; Ma, Jun Y.Ing; Tang, Yuhong; Taylor, Mark; Roossinck, Marilyn J.

In: Molecular Plant-Microbe Interactions, Vol. 25, No. 8, 01.08.2012, p. 1034-1044.

Research output: Contribution to journalArticle

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AU - Xu, Ping

AU - Wang, Hua

AU - Coker, Frank

AU - Ma, Jun Y.Ing

AU - Tang, Yuhong

AU - Taylor, Mark

AU - Roossinck, Marilyn J.

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