Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata

Yan Wang, Xi Wang, Andrew G. McCubbin, Teh Hui Kao

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multigene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.

Original languageEnglish (US)
Pages (from-to)565-580
Number of pages16
JournalPlant molecular biology
Volume53
Issue number4
DOIs
StatePublished - Nov 1 2003

Fingerprint

Petunia integrifolia
Petunia
chromosome mapping
Molecular Biology
loci
Genes
A 113
genes
Pollen
Genetic Recombination
pollen
Norgestrienone
Chromosome Walking
chromosome walking
Solanaceae
suppression subtractive hybridization
Gene Expression Profiling
Restriction Fragment Length Polymorphisms
Introns
Haplotypes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

@article{33e2f0258b464bbda4bfbf6452e6433b,
title = "Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata",
abstract = "Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multigene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.",
author = "Yan Wang and Xi Wang and McCubbin, {Andrew G.} and Kao, {Teh Hui}",
year = "2003",
month = "11",
day = "1",
doi = "10.1023/B:PLAN.0000019068.00034.09",
language = "English (US)",
volume = "53",
pages = "565--580",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "4",

}

Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata. / Wang, Yan; Wang, Xi; McCubbin, Andrew G.; Kao, Teh Hui.

In: Plant molecular biology, Vol. 53, No. 4, 01.11.2003, p. 565-580.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata

AU - Wang, Yan

AU - Wang, Xi

AU - McCubbin, Andrew G.

AU - Kao, Teh Hui

PY - 2003/11/1

Y1 - 2003/11/1

N2 - Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multigene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.

AB - Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multigene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.

UR - http://www.scopus.com/inward/record.url?scp=1542580431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542580431&partnerID=8YFLogxK

U2 - 10.1023/B:PLAN.0000019068.00034.09

DO - 10.1023/B:PLAN.0000019068.00034.09

M3 - Article

C2 - 15010619

AN - SCOPUS:1542580431

VL - 53

SP - 565

EP - 580

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 4

ER -