Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast

S. Carl Falco, Yuyang Li, James Broach, David Botstein

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We obtained strains of yeast with large segments of 2μ plasmid DNA integrated at several chromosomal locations by selecting genetically for recombination between a chromosomal sequence carried on a 2μ-circle-containing hybrid plasmid and a homologous sequence on the chromosome. In all diploids examined, the presence of 2μ circle sequences causes a marked instability of the chromosome into which the 2μ DNA is inserted. Although in some cases the loss of genetic markers is due to physical loss of the entire chromosome, in most cases the loss of markers appears to be due to a mitotic homozygotization of markers: the allelic information from the homologous chromosome replaces the information distal to the integrated 2μ DNA. The instability caused by integrated 2μ DNA sequences requires the activity of the specialized site-specific recombination system encoded by the 2μ plasmid. We propose that the presence of integrated 2μ DNA allows efficient integration of additional copies of the intact 2μ plasmid by the action of the plasmid-coded special recombination system. Unequal sister-strand exchanges within the inverted repetition would result in the formation of dicentric chromosomes whose breakage during mitosis might begin a cycle analogous to the breakage-fusion-bridge cycle described many years ago in maize.

Original languageEnglish (US)
Pages (from-to)573-584
Number of pages12
JournalCell
Volume29
Issue number2
DOIs
StatePublished - Jan 1 1982

Fingerprint

Chromosomes
Yeast
Plasmids
Yeasts
DNA
Genetic Recombination
Chromosome Breakage
Chromosomal Instability
DNA sequences
Sequence Homology
Diploidy
Genetic Markers
Mitosis
Zea mays
Fusion reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Falco, S. Carl ; Li, Yuyang ; Broach, James ; Botstein, David. / Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast. In: Cell. 1982 ; Vol. 29, No. 2. pp. 573-584.
@article{3480c7562ab94dc7815d4f40602c6521,
title = "Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast",
abstract = "We obtained strains of yeast with large segments of 2μ plasmid DNA integrated at several chromosomal locations by selecting genetically for recombination between a chromosomal sequence carried on a 2μ-circle-containing hybrid plasmid and a homologous sequence on the chromosome. In all diploids examined, the presence of 2μ circle sequences causes a marked instability of the chromosome into which the 2μ DNA is inserted. Although in some cases the loss of genetic markers is due to physical loss of the entire chromosome, in most cases the loss of markers appears to be due to a mitotic homozygotization of markers: the allelic information from the homologous chromosome replaces the information distal to the integrated 2μ DNA. The instability caused by integrated 2μ DNA sequences requires the activity of the specialized site-specific recombination system encoded by the 2μ plasmid. We propose that the presence of integrated 2μ DNA allows efficient integration of additional copies of the intact 2μ plasmid by the action of the plasmid-coded special recombination system. Unequal sister-strand exchanges within the inverted repetition would result in the formation of dicentric chromosomes whose breakage during mitosis might begin a cycle analogous to the breakage-fusion-bridge cycle described many years ago in maize.",
author = "Falco, {S. Carl} and Yuyang Li and James Broach and David Botstein",
year = "1982",
month = "1",
day = "1",
doi = "10.1016/0092-8674(82)90173-8",
language = "English (US)",
volume = "29",
pages = "573--584",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast. / Falco, S. Carl; Li, Yuyang; Broach, James; Botstein, David.

In: Cell, Vol. 29, No. 2, 01.01.1982, p. 573-584.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast

AU - Falco, S. Carl

AU - Li, Yuyang

AU - Broach, James

AU - Botstein, David

PY - 1982/1/1

Y1 - 1982/1/1

N2 - We obtained strains of yeast with large segments of 2μ plasmid DNA integrated at several chromosomal locations by selecting genetically for recombination between a chromosomal sequence carried on a 2μ-circle-containing hybrid plasmid and a homologous sequence on the chromosome. In all diploids examined, the presence of 2μ circle sequences causes a marked instability of the chromosome into which the 2μ DNA is inserted. Although in some cases the loss of genetic markers is due to physical loss of the entire chromosome, in most cases the loss of markers appears to be due to a mitotic homozygotization of markers: the allelic information from the homologous chromosome replaces the information distal to the integrated 2μ DNA. The instability caused by integrated 2μ DNA sequences requires the activity of the specialized site-specific recombination system encoded by the 2μ plasmid. We propose that the presence of integrated 2μ DNA allows efficient integration of additional copies of the intact 2μ plasmid by the action of the plasmid-coded special recombination system. Unequal sister-strand exchanges within the inverted repetition would result in the formation of dicentric chromosomes whose breakage during mitosis might begin a cycle analogous to the breakage-fusion-bridge cycle described many years ago in maize.

AB - We obtained strains of yeast with large segments of 2μ plasmid DNA integrated at several chromosomal locations by selecting genetically for recombination between a chromosomal sequence carried on a 2μ-circle-containing hybrid plasmid and a homologous sequence on the chromosome. In all diploids examined, the presence of 2μ circle sequences causes a marked instability of the chromosome into which the 2μ DNA is inserted. Although in some cases the loss of genetic markers is due to physical loss of the entire chromosome, in most cases the loss of markers appears to be due to a mitotic homozygotization of markers: the allelic information from the homologous chromosome replaces the information distal to the integrated 2μ DNA. The instability caused by integrated 2μ DNA sequences requires the activity of the specialized site-specific recombination system encoded by the 2μ plasmid. We propose that the presence of integrated 2μ DNA allows efficient integration of additional copies of the intact 2μ plasmid by the action of the plasmid-coded special recombination system. Unequal sister-strand exchanges within the inverted repetition would result in the formation of dicentric chromosomes whose breakage during mitosis might begin a cycle analogous to the breakage-fusion-bridge cycle described many years ago in maize.

UR - http://www.scopus.com/inward/record.url?scp=0019970245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019970245&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(82)90173-8

DO - 10.1016/0092-8674(82)90173-8

M3 - Article

C2 - 6288264

AN - SCOPUS:0019970245

VL - 29

SP - 573

EP - 584

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -