Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene

Zhaoqing Yang, Jun Miao, Yaming Huang, Xinyi Li, Chaturong Putaporntip, Somchai Jongwutiwes, Qi Gao, Rachanee Udomsangpetch, Jetsumon Sattabongkot, Liwang Cui

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5% in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6% of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalActa Tropica
Volume100
Issue number3
DOIs
StatePublished - Dec 1 2006

Fingerprint

Plasmodium vivax
Merozoites
merozoites
Genetic Structures
surface proteins
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
Membrane Proteins
Parasites
polymerase chain reaction
Genotype
Polymerase Chain Reaction
Population
Genes
genes
parasites
Epidemiological Monitoring
Vivax Malaria
genotype
Thailand

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Yang, Zhaoqing ; Miao, Jun ; Huang, Yaming ; Li, Xinyi ; Putaporntip, Chaturong ; Jongwutiwes, Somchai ; Gao, Qi ; Udomsangpetch, Rachanee ; Sattabongkot, Jetsumon ; Cui, Liwang. / Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene. In: Acta Tropica. 2006 ; Vol. 100, No. 3. pp. 205-212.
@article{62bd0006e4704bdb9d9501616aac5012,
title = "Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene",
abstract = "The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5{\%} in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6{\%} of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections.",
author = "Zhaoqing Yang and Jun Miao and Yaming Huang and Xinyi Li and Chaturong Putaporntip and Somchai Jongwutiwes and Qi Gao and Rachanee Udomsangpetch and Jetsumon Sattabongkot and Liwang Cui",
year = "2006",
month = "12",
day = "1",
doi = "10.1016/j.actatropica.2006.10.011",
language = "English (US)",
volume = "100",
pages = "205--212",
journal = "Acta Tropica",
issn = "0001-706X",
publisher = "Elsevier",
number = "3",

}

Yang, Z, Miao, J, Huang, Y, Li, X, Putaporntip, C, Jongwutiwes, S, Gao, Q, Udomsangpetch, R, Sattabongkot, J & Cui, L 2006, 'Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene', Acta Tropica, vol. 100, no. 3, pp. 205-212. https://doi.org/10.1016/j.actatropica.2006.10.011

Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene. / Yang, Zhaoqing; Miao, Jun; Huang, Yaming; Li, Xinyi; Putaporntip, Chaturong; Jongwutiwes, Somchai; Gao, Qi; Udomsangpetch, Rachanee; Sattabongkot, Jetsumon; Cui, Liwang.

In: Acta Tropica, Vol. 100, No. 3, 01.12.2006, p. 205-212.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene

AU - Yang, Zhaoqing

AU - Miao, Jun

AU - Huang, Yaming

AU - Li, Xinyi

AU - Putaporntip, Chaturong

AU - Jongwutiwes, Somchai

AU - Gao, Qi

AU - Udomsangpetch, Rachanee

AU - Sattabongkot, Jetsumon

AU - Cui, Liwang

PY - 2006/12/1

Y1 - 2006/12/1

N2 - The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5% in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6% of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections.

AB - The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5% in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6% of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections.

UR - http://www.scopus.com/inward/record.url?scp=33846581910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846581910&partnerID=8YFLogxK

U2 - 10.1016/j.actatropica.2006.10.011

DO - 10.1016/j.actatropica.2006.10.011

M3 - Article

VL - 100

SP - 205

EP - 212

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

IS - 3

ER -