Variabilidade genśtica de isolados Brasileiros de Alternaria alternata por meio de marcadores moleculares de AFLP e RAPD

Translated title of the contribution: Genetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques

Francisco Dini-Andreote, Vivian Cristina Pietrobon, Fernando Dini Andreote, Aline Silva Romão, Marcel Bellato Spósito, Welington Luiz Araújo

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The Alternaria brown spot (ABS) is a disease caused in tangerine plants and its hybrids by the fungus Alternaria alternata f. sp. citri which has been found in Brazil since 2001. Due to the recent occurrence in Brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. Here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of A. alternata f. sp. citri from citrus plants and four endophytic isolates from mango (one Alternaria tenuissima and three Alternaria arborescens). The application of two molecular markers Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) had revealed the isolates clustering in distinct groups when fingerprintings were analyzed by Principal Components Analysis (PCA). Despite the better assessment of the genetic variability through the AFLP, significant modifications in clusters components were not observed, and only slight shifts in the positioning of isolates LRS 39/3 and 25M were observed in PCA plots. Furthermore, in both analyses, only the isolates from lemon plants revealed to be clustered, differently from the absence of clustering for other hosts or plant tissues. Summarizing, both RAPD and AFLP analyses were both efficient to detect the genetic variability within the population of the pathogenic fungus Alternaria spp., supplying information on the genetic variability of this species as a basis for further studies aiming the disease control.

Translated title of the contributionGenetic variability of Brazilian isolates of Alternaria alternata detected by AFLP and RAPD techniques
Original languagePortuguese
Pages (from-to)670-677
Number of pages8
JournalBrazilian Journal of Microbiology
Volume40
Issue number3
DOIs
StatePublished - 2009

All Science Journal Classification (ASJC) codes

  • Microbiology

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