Genetic variation within clonal lineages of phytophthora infestans revealed through genotyping-by-sequencing, and implications for late blight epidemiology

Zachariah R. Hansen, Kathryne L. Everts, William E. Fry, Amanda J. Gevens, Niklaus J. Grünwald, Beth K. Gugino, Dennis A. Johnson, Steven B. Johnson, Howard S. Judelson, Brian J. Knaus, Margaret T. McGrath, Kevin L. Myers, Jean B. Ristaino, Pamela D. Roberts, Gary A. Secor, Christine D. Smart

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Abstract

Genotyping-by-sequencing (GBS) was performed on 257 Phytophthora infestans isolates belonging to four clonal lineages to study within-lineage diversity. The four lineages used in the study were US-8 (n = 28), US-11 (n = 27), US-23 (n = 166), and US-24 (n = 36), with isolates originating from 23 of the United States and Ontario, Canada. The majority of isolates were collected between 2010 and 2014 (94%), with the remaining isolates collected from 1994 to 2009, and 2015. Between 3,774 and 5,070 single-nucleotide polymorphisms (SNPs) were identified within each lineage and were used to investigate relationships among individuals. K-means hierarchical clustering revealed three clusters within lineage US-23, with US-23 isolates clustering more by collection year than by geographic origin. Kmeans hierarchical clustering did not reveal significant clustering within the smaller US-8, US-11, and US-24 data sets. Neighbor-joining (NJ) trees were also constructed for each lineage. All four NJ trees revealed evidence for pathogen dispersal and overwintering within regions, as well as long-distance pathogen transport across regions. In the US-23 NJ tree, grouping by year was more prominent than grouping by region, which indicates the importance of long-distance pathogen transport as a source of initial late blight inoculum. Our results support previous studies that found significant genetic diversity within clonal lineages of P. infestans and show that GBS offers sufficiently high resolution to detect substructuring within clonal populations.

Original languageEnglish (US)
Article numbere0165690
JournalPloS one
Volume11
Issue number11
DOIs
StatePublished - Nov 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Hansen, Z. R., Everts, K. L., Fry, W. E., Gevens, A. J., Grünwald, N. J., Gugino, B. K., Johnson, D. A., Johnson, S. B., Judelson, H. S., Knaus, B. J., McGrath, M. T., Myers, K. L., Ristaino, J. B., Roberts, P. D., Secor, G. A., & Smart, C. D. (2016). Genetic variation within clonal lineages of phytophthora infestans revealed through genotyping-by-sequencing, and implications for late blight epidemiology. PloS one, 11(11), [e0165690]. https://doi.org/10.1371/journal.pone.0165690