Genome wide copy number analysis of paediatric Burkitt lymphoma using formalin-fixed tissues reveals a subset with gain of chromosome 13q and corresponding miRNA over expression

Joshua D. Schiffman, Patrick D. Lorimer, Vladimir Rodic, Mona S. Jahromi, Jonathan M. Downie, Michael G. Bayerl, Jennifer N. Sanmann, Pamela A. Althof, Warren G. Sanger, Phillip Barnette, Sherrie L. Perkins, Rodney R. Miles

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41 Scopus citations

Abstract

The majority of paediatric Burkitt lymphoma (pBL) patients that relapse will die of disease, but markers for this high-risk subset are unknown. MYC translocations characterize pBL, but additional genetic changes may relate to prognosis and serve as potential biomarkers. We utilized a molecular inversion probe single nucleotide polymorphism assay to perform high resolution, genome-wide copy number analysis on archival formalin-fixed, paraffin-embedded pBL and germline tissues. We identified copy number abnormalities (CNAs) in 18/28 patients (64%) with a total of 62 CNAs that included 32 gains and 30 copy number losses. We identified seven recurrent CNAs including 1q gain (7/28, 25%), 13q gain (3/28, 11%), and 17p loss (4/28, 14%). The minimum common amplified region on 13q was at 13q31 and included the MIR17HG (MIR17-92) locus. Samples with this gain had higher levels of MIR17 RNA and showed a tendency for early relapse. Tumour-specific uniparental disomy was identified in 32% of cases and usually was recurrent. These results demonstrate that high-resolution copy number analysis can be performed on archival lymphoma tissue specimens, which has significance for the study of rare diseases.

Original languageEnglish (US)
Pages (from-to)477-486
Number of pages10
JournalBritish Journal of Haematology
Volume155
Issue number4
DOIs
StatePublished - Nov 2011

All Science Journal Classification (ASJC) codes

  • Hematology

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    Schiffman, J. D., Lorimer, P. D., Rodic, V., Jahromi, M. S., Downie, J. M., Bayerl, M. G., Sanmann, J. N., Althof, P. A., Sanger, W. G., Barnette, P., Perkins, S. L., & Miles, R. R. (2011). Genome wide copy number analysis of paediatric Burkitt lymphoma using formalin-fixed tissues reveals a subset with gain of chromosome 13q and corresponding miRNA over expression. British Journal of Haematology, 155(4), 477-486. https://doi.org/10.1111/j.1365-2141.2011.08883.x