Genome-wide function of H2B ubiquitylation in promoter and genic regions

Kiran Batta, Zhenhai Zhang, Kuangyu Yen, David B. Goffman, B. Franklin Pugh

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

Nucleosomal organization in and around genes may contribute substantially to transcriptional regulation. The contribution of histone modifications to genome-wide nucleosomal organization has not been systematically evaluated. In the present study, we examine the role of H2BK123 ubiquitylation, a key regulator of several histone modifications, on nucleosomal organization at promoter, genic, and transcription termination regions in Saccharomyces cerevisiae. Using high-resolutionMNase chromatin immunoprecipitation and sequencing (ChIP-seq), we map nucleosome positioning and occupancy in mutants of the H2BK123 ubiquitylation pathway. We found that H2B ubiquitylation-mediated nucleosome formation and/or stability inhibits the assembly of the transcription machinery at normally quiescent promoters, whereas ubiquitylation within highly active gene bodies promotes transcription elongation. This regulation does not proceed through ubiquitylation-regulated histone marks at H3K4, K36, and K79. Our findings suggest that mechanistically similar functions of H2B ubiquitylation (nucleosome assembly) elicit different functional outcomes on genes depending on its positional context in promoters (repressive) versus transcribed regions (activating).

Original languageEnglish (US)
Pages (from-to)2254-2265
Number of pages12
JournalGenes and Development
Volume25
Issue number21
DOIs
StatePublished - Nov 1 2011

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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    Batta, K., Zhang, Z., Yen, K., Goffman, D. B., & Franklin Pugh, B. (2011). Genome-wide function of H2B ubiquitylation in promoter and genic regions. Genes and Development, 25(21), 2254-2265. https://doi.org/10.1101/gad.177238.111