Genome-wide mapping of nucleosome positions in yeast using high-resolution MNase ChIP-Seq

Megha Wal, B. Franklin Pugh

Research output: Chapter in Book/Report/Conference proceedingChapter

30 Scopus citations

Abstract

Eukaryotic DNA is packaged into chromatin where nucleosomes form the basic building unit. Knowing the precise positions of nucleosomes is important because they determine the accessibility of underlying regulatory DNA sequences. Here we describe a detailed method to map on a genomic scale the locations of nucleosomes with very high resolution. Micrococcal nuclease (MNase) digestion followed by chromatin immunoprecipitation and facilitated library construction for deep sequencing provides a simple and accurate map of nucleosome positions.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages233-250
Number of pages18
DOIs
StatePublished - 2012

Publication series

NameMethods in Enzymology
Volume513
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Wal, M., & Pugh, B. F. (2012). Genome-wide mapping of nucleosome positions in yeast using high-resolution MNase ChIP-Seq. In Methods in Enzymology (pp. 233-250). (Methods in Enzymology; Vol. 513). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-391938-0.00010-0