Genomic characterization of a turkey reovirus field strain by Next-Generation Sequencing

Yi Tang, Huaguang Lu, Aswathy Sebastian, Yin Ting Yeh, Craig A. Praul, Istvan U. Albert, Si Yang Zheng

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496. bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192. bp (S4) to 3959. bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalInfection, Genetics and Evolution
Volume32
DOIs
StatePublished - Jun 1 2015

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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