Genomic Deoxyribonucleic Acid Restriction Fragment Length Polymorphism of Streptococcus uberis

Evidence of Clonal Diversity

Bhushan M. Jayarao, E. E. Schilling, S. P. Oliver

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fifty plasmid-free strains of Streptococcus uberis, including 40 strains isolated from cows in two dairy herds in Tennessee with subclinical and clinical mastitis, 9 strains isolated from cows in the US, and 1 strain from Great Britain were examined for genomic DNA restriction fragment length polymorphisms. The 50 strains belonged to 35 DNA fingerprint patterns. Within a herd, fingerprint patterns from isolates obtained from cows with subclinical mastitis at different periods of the lactation cycle and from episodes of clinical mastitis were similar. Restriction fragment length polymorphism analysis revealed the presence of DNA fragments ≥21 kb in all 12 strains of S. uberis isolated from episodes of clinical mastitis and in only 8 of the 38 (21%) remaining strains. Cluster analysis placed the 50 strains into eight distinct clusters. Most strains from the northeastern US were confined to one cluster, whereas most of the isolates from episodes of clinical mastitis belonged to two different clusters. These data suggest that clonal diversity exists among strains of S. uberis isolated from bovine mammary secretions.

Original languageEnglish (US)
Pages (from-to)468-474
Number of pages7
JournalJournal of dairy science
Volume76
Issue number2
DOIs
StatePublished - Jan 1 1993

Fingerprint

Streptococcus uberis
Mastitis
Streptococcus
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
genomics
DNA
mastitis
DNA Fingerprinting
Dermatoglyphics
Lactation
cows
Cluster Analysis
Breast
Plasmids
DNA fingerprinting
dairy herds
breasts
cluster analysis
plasmids

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

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abstract = "Fifty plasmid-free strains of Streptococcus uberis, including 40 strains isolated from cows in two dairy herds in Tennessee with subclinical and clinical mastitis, 9 strains isolated from cows in the US, and 1 strain from Great Britain were examined for genomic DNA restriction fragment length polymorphisms. The 50 strains belonged to 35 DNA fingerprint patterns. Within a herd, fingerprint patterns from isolates obtained from cows with subclinical mastitis at different periods of the lactation cycle and from episodes of clinical mastitis were similar. Restriction fragment length polymorphism analysis revealed the presence of DNA fragments ≥21 kb in all 12 strains of S. uberis isolated from episodes of clinical mastitis and in only 8 of the 38 (21{\%}) remaining strains. Cluster analysis placed the 50 strains into eight distinct clusters. Most strains from the northeastern US were confined to one cluster, whereas most of the isolates from episodes of clinical mastitis belonged to two different clusters. These data suggest that clonal diversity exists among strains of S. uberis isolated from bovine mammary secretions.",
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Genomic Deoxyribonucleic Acid Restriction Fragment Length Polymorphism of Streptococcus uberis : Evidence of Clonal Diversity. / Jayarao, Bhushan M.; Schilling, E. E.; Oliver, S. P.

In: Journal of dairy science, Vol. 76, No. 2, 01.01.1993, p. 468-474.

Research output: Contribution to journalArticle

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