Genomic homologous recombination in planta

Susannah Gal, B. Pisan, T. Hohn, N. Grimsley, B. Hohn

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A system for monitoring intrachromosomal homologous recombination in whole plants is described. A multimer of cauliflower mosaic virus (CaMV) sequences, arranged such that CaMV could only be produced by recombination, was integrated into Brassica napus nuclear DNA. This set-up allowed scoring of recombination events by the appearance of viral symptoms. The repeated homologous regions were derived from two different strains of CaMV so that different recombinant viruses (i.e. different recombination events) could be distinguished. In most of the transgenic plants, a single major virus species was detected. About half of the transgenic plants contained viruses of the same type, suggesting a hotspot for recombination. The remainder of the plants contained viruses with cross-over sites distributed throughout the rest of the homologous sequence. Sequence analysis of two recombinant molecules suggests that mismatch repair is linked to the recombination process.

Original languageEnglish (US)
Pages (from-to)1571-1578
Number of pages8
JournalEMBO Journal
Volume10
Issue number6
StatePublished - May 23 1991

Fingerprint

Homologous Recombination
Viruses
Genetic Recombination
Caulimovirus
Plant Viruses
Genetically Modified Plants
Brassica napus
DNA Mismatch Repair
Sequence Homology
Sequence Analysis
Repair
DNA
Molecules
Monitoring

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Gal, S., Pisan, B., Hohn, T., Grimsley, N., & Hohn, B. (1991). Genomic homologous recombination in planta. EMBO Journal, 10(6), 1571-1578.
Gal, Susannah ; Pisan, B. ; Hohn, T. ; Grimsley, N. ; Hohn, B. / Genomic homologous recombination in planta. In: EMBO Journal. 1991 ; Vol. 10, No. 6. pp. 1571-1578.
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Gal, S, Pisan, B, Hohn, T, Grimsley, N & Hohn, B 1991, 'Genomic homologous recombination in planta', EMBO Journal, vol. 10, no. 6, pp. 1571-1578.

Genomic homologous recombination in planta. / Gal, Susannah; Pisan, B.; Hohn, T.; Grimsley, N.; Hohn, B.

In: EMBO Journal, Vol. 10, No. 6, 23.05.1991, p. 1571-1578.

Research output: Contribution to journalArticle

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Gal S, Pisan B, Hohn T, Grimsley N, Hohn B. Genomic homologous recombination in planta. EMBO Journal. 1991 May 23;10(6):1571-1578.