Genomic strategies for the identification of dopamine receptor genes in zebrafish

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

In this chapter, we describe the identification and cloning of D2-like dopamine receptor (DR) genes in zebrafish, a vertebrate model genetic organism. To identify DR genes, we performed searches of the zebrafish genomic sequence database that yielded contig segments of several D2-like DR genes. From these sequences, we amplified full-length cDNAs encoding three D2, one D3, and three D4 DR receptor subtypes via RT-PCR. The predicted proteins displayed 57-72% amino acid identity when compared to their human DR counterparts. To validate the identity of zebrafish DR genes, each of the genes was mapped by using the T51 radiation hybrid panel. With the exception of drd2b and drd4b, each of the zebrafish DR genes mapped to chromosomal positions that were syntenic with regions of human chromosomes containing orthologs of the zebrafish DR genes. To further validate the identity of the D2-like DR genes in zebrafish, we conducted phylogenetic analysis which supported the predicted identities of the cloned DR receptor cDNAs.

Original languageEnglish (US)
Title of host publicationDopamine
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages201-214
Number of pages14
ISBN (Print)9781627032506
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume964
ISSN (Print)1064-3745

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Boehmler, W., Petko, J., Canfield, V. A., & Levenson, R. (2013). Genomic strategies for the identification of dopamine receptor genes in zebrafish. In Dopamine: Methods and Protocols (pp. 201-214). (Methods in Molecular Biology; Vol. 964). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-251-3_12