Geographic distribution and rDNA-ITS region sequence diversity of Waitea circinata var.circinata isolated from annual bluegrass in the United States

Chi Min Chen, Karla A. De La Cerda, John Edward Kaminski, III, Greg W. Douhan, Frances P. Wong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Waitea circinata var. circinata is the causal agent of brown ring patch, an emergent disease of turfgrass in the United States. Forty-two isolates from annual bluegrass were obtained from California, Connecticut, Idaho, Illinois, Massachusetts, New York, Ohio, Oregon, and Rhode Island. Almost all isolates produced white to orange sclerotia (bulbils), 2 to 5 mm in size, that turned dark brown after 21 days on 1/4-strength potato dextrose agar. The ribosomal DNA internal transcribed spacer regions and 5.8S region (ITS) were analyzed by restriction fragment length polymorphism (RFLP) analysis using MspI and sequencing to attempt identification of the isolates. Some isolates were heterozygous at the MspI restriction site, results not found in previous reports using the RFLP technique for identification. Four additional nucleotide positions were found to be variable within ITS based on sequence analysis, including two indels and two additional heterozygous positions. A total of 17 ITS haplotypes were found, and there was no obvious relationship between ITS haplotype and the geographic distribution of the isolates. Results of this work indicate that W. circinata var. circinata is present in multiple states and provide an initial understanding of the diversity of the pathogen in the United States.

Original languageEnglish (US)
Pages (from-to)906-911
Number of pages6
JournalPlant disease
Volume93
Issue number9
DOIs
StatePublished - Sep 1 2009

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Poa annua
haplotypes
geographical distribution
restriction fragment length polymorphism
sclerotia
emerging diseases
turf grasses
ribosomal DNA
internal transcribed spacers
sequence analysis
agar
nucleotides
potatoes
glucose
pathogens
sequence diversity
methodology

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Agronomy and Crop Science

Cite this

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title = "Geographic distribution and rDNA-ITS region sequence diversity of Waitea circinata var.circinata isolated from annual bluegrass in the United States",
abstract = "Waitea circinata var. circinata is the causal agent of brown ring patch, an emergent disease of turfgrass in the United States. Forty-two isolates from annual bluegrass were obtained from California, Connecticut, Idaho, Illinois, Massachusetts, New York, Ohio, Oregon, and Rhode Island. Almost all isolates produced white to orange sclerotia (bulbils), 2 to 5 mm in size, that turned dark brown after 21 days on 1/4-strength potato dextrose agar. The ribosomal DNA internal transcribed spacer regions and 5.8S region (ITS) were analyzed by restriction fragment length polymorphism (RFLP) analysis using MspI and sequencing to attempt identification of the isolates. Some isolates were heterozygous at the MspI restriction site, results not found in previous reports using the RFLP technique for identification. Four additional nucleotide positions were found to be variable within ITS based on sequence analysis, including two indels and two additional heterozygous positions. A total of 17 ITS haplotypes were found, and there was no obvious relationship between ITS haplotype and the geographic distribution of the isolates. Results of this work indicate that W. circinata var. circinata is present in multiple states and provide an initial understanding of the diversity of the pathogen in the United States.",
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Geographic distribution and rDNA-ITS region sequence diversity of Waitea circinata var.circinata isolated from annual bluegrass in the United States. / Chen, Chi Min; De La Cerda, Karla A.; Kaminski, III, John Edward; Douhan, Greg W.; Wong, Frances P.

In: Plant disease, Vol. 93, No. 9, 01.09.2009, p. 906-911.

Research output: Contribution to journalArticle

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