Germline repertoire of the immunoglobulin V(H)3 family in rhesus monkeys

Erika F. H. Saunders, Norman L. Letvin, David H. Margolin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To facilitate molecular studies of antibody responses in rhesus monkeys (Macaca mulatta), we cloned and sequenced germline segments from its largest and most diverse immunoglobulin heavy-chain gene family, V(H)3. Using a PCR-based approach, we characterized 29 sequences, 20 with open reading frames (ORFs) and 9 pseudogenes. The leader sequences, introns, exons, and recombination signal sequences of M. mulatta V(H)3 gene segments are not strictly identical to those of humans, but the mature coding regions demonstrate, on average, greater than 90% sequence similarity. Although the framework regions are more highly conserved, the complementarity-determining regions (CDRs) also show strong similarities, and their predicted three-dimensional structures resemble those of their human homologues. In one instance, homologous macaque and human CDR1 sequences were 100% identical at the nucleotide level, and some CDR2s shared nucleotide identity as high as 96.5%. However, some rhesus V(H)3 ORFs have unusual structural features, including atypical CDR lengths and uncommon amino acids at structurally crucial positions. The similarity of rhesus and human V(H)3 homologues reinforces the notion that humoral immunity in this nonhuman primate species is an appropriate system for modeling human antibody responses.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalImmunogenetics
Volume51
Issue number7
DOIs
StatePublished - Jul 3 2000

Fingerprint

Immunoglobulin Variable Region
Macaca mulatta
Complementarity Determining Regions
Open Reading Frames
Antibody Formation
Nucleotides
Immunoglobulin Heavy Chain Genes
Pseudogenes
Macaca
Humoral Immunity
Protein Sorting Signals
Introns
Primates
Genetic Recombination
Exons
Amino Acids
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Saunders, Erika F. H. ; Letvin, Norman L. ; Margolin, David H. / Germline repertoire of the immunoglobulin V(H)3 family in rhesus monkeys. In: Immunogenetics. 2000 ; Vol. 51, No. 7. pp. 519-527.
@article{c4b7166862f348d79ddde0aa0e77bc08,
title = "Germline repertoire of the immunoglobulin V(H)3 family in rhesus monkeys",
abstract = "To facilitate molecular studies of antibody responses in rhesus monkeys (Macaca mulatta), we cloned and sequenced germline segments from its largest and most diverse immunoglobulin heavy-chain gene family, V(H)3. Using a PCR-based approach, we characterized 29 sequences, 20 with open reading frames (ORFs) and 9 pseudogenes. The leader sequences, introns, exons, and recombination signal sequences of M. mulatta V(H)3 gene segments are not strictly identical to those of humans, but the mature coding regions demonstrate, on average, greater than 90{\%} sequence similarity. Although the framework regions are more highly conserved, the complementarity-determining regions (CDRs) also show strong similarities, and their predicted three-dimensional structures resemble those of their human homologues. In one instance, homologous macaque and human CDR1 sequences were 100{\%} identical at the nucleotide level, and some CDR2s shared nucleotide identity as high as 96.5{\%}. However, some rhesus V(H)3 ORFs have unusual structural features, including atypical CDR lengths and uncommon amino acids at structurally crucial positions. The similarity of rhesus and human V(H)3 homologues reinforces the notion that humoral immunity in this nonhuman primate species is an appropriate system for modeling human antibody responses.",
author = "Saunders, {Erika F. H.} and Letvin, {Norman L.} and Margolin, {David H.}",
year = "2000",
month = "7",
day = "3",
doi = "10.1007/s002510000170",
language = "English (US)",
volume = "51",
pages = "519--527",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "7",

}

Germline repertoire of the immunoglobulin V(H)3 family in rhesus monkeys. / Saunders, Erika F. H.; Letvin, Norman L.; Margolin, David H.

In: Immunogenetics, Vol. 51, No. 7, 03.07.2000, p. 519-527.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Germline repertoire of the immunoglobulin V(H)3 family in rhesus monkeys

AU - Saunders, Erika F. H.

AU - Letvin, Norman L.

AU - Margolin, David H.

PY - 2000/7/3

Y1 - 2000/7/3

N2 - To facilitate molecular studies of antibody responses in rhesus monkeys (Macaca mulatta), we cloned and sequenced germline segments from its largest and most diverse immunoglobulin heavy-chain gene family, V(H)3. Using a PCR-based approach, we characterized 29 sequences, 20 with open reading frames (ORFs) and 9 pseudogenes. The leader sequences, introns, exons, and recombination signal sequences of M. mulatta V(H)3 gene segments are not strictly identical to those of humans, but the mature coding regions demonstrate, on average, greater than 90% sequence similarity. Although the framework regions are more highly conserved, the complementarity-determining regions (CDRs) also show strong similarities, and their predicted three-dimensional structures resemble those of their human homologues. In one instance, homologous macaque and human CDR1 sequences were 100% identical at the nucleotide level, and some CDR2s shared nucleotide identity as high as 96.5%. However, some rhesus V(H)3 ORFs have unusual structural features, including atypical CDR lengths and uncommon amino acids at structurally crucial positions. The similarity of rhesus and human V(H)3 homologues reinforces the notion that humoral immunity in this nonhuman primate species is an appropriate system for modeling human antibody responses.

AB - To facilitate molecular studies of antibody responses in rhesus monkeys (Macaca mulatta), we cloned and sequenced germline segments from its largest and most diverse immunoglobulin heavy-chain gene family, V(H)3. Using a PCR-based approach, we characterized 29 sequences, 20 with open reading frames (ORFs) and 9 pseudogenes. The leader sequences, introns, exons, and recombination signal sequences of M. mulatta V(H)3 gene segments are not strictly identical to those of humans, but the mature coding regions demonstrate, on average, greater than 90% sequence similarity. Although the framework regions are more highly conserved, the complementarity-determining regions (CDRs) also show strong similarities, and their predicted three-dimensional structures resemble those of their human homologues. In one instance, homologous macaque and human CDR1 sequences were 100% identical at the nucleotide level, and some CDR2s shared nucleotide identity as high as 96.5%. However, some rhesus V(H)3 ORFs have unusual structural features, including atypical CDR lengths and uncommon amino acids at structurally crucial positions. The similarity of rhesus and human V(H)3 homologues reinforces the notion that humoral immunity in this nonhuman primate species is an appropriate system for modeling human antibody responses.

UR - http://www.scopus.com/inward/record.url?scp=0034124751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034124751&partnerID=8YFLogxK

U2 - 10.1007/s002510000170

DO - 10.1007/s002510000170

M3 - Article

C2 - 10912503

AN - SCOPUS:0034124751

VL - 51

SP - 519

EP - 527

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 7

ER -