Gestation alters the gut microbiota of an oviparous lizard

Brian K. Trevelline, Kirsty J. MacLeod, Tracy Lee Langkilde, Kevin D. Kohl

Research output: Contribution to journalArticle

Abstract

Mammalian pregnancy can alter the diversity, membership and structure of the maternal gut microbiota, but it is unclear whether this phenomenon occurs in vertebrates with different reproductive strategies. We conducted 16S rRNA bacterial inventories to investigate whether oviparous lizards exhibit shifts in gut microbiota similar to those observed in mammals. Using wild-caught eastern fence lizards from Alabama, USA, we collected and extracted fecal DNA from gravid and non-gravid individuals over 54 days in captivity. We predicted that, like mammals, the alpha diversity of lizard gut microbiota would decrease over gestation, and that inter-individual variation in community composition would increase. Indeed, we found that individuals in late-gestation harbored lower gut bacterial richness compared to non-gravid females. Lizard gut microbial communities of late-gestational females exhibited higher pairwise distances for both community membership and community structure compared to earlier gestation stages, indicating a higher degree of inter-individual variation as gestation progressed. Additionally, we found that the relative abundance and prevalence of the candidate phylum Melainabacteria tended to decrease over the course of gestation. While the consequences of these specific alterations are unknown, our results suggest that a general restructuring of gut microbial communities over gestation may be widespread across vertebrate reproductive strategies.

Original languageEnglish (US)
JournalFEMS microbiology ecology
Volume95
Issue number7
DOIs
StatePublished - Jul 1 2019

Fingerprint

Oviparity
Lizards
pregnancy
lizard
Pregnancy
individual variation
reproductive strategy
Vertebrates
microbial community
Mammals
vertebrate
mammal
captivity
Gastrointestinal Microbiome
community composition
relative abundance
community structure
Mothers
DNA
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Trevelline, Brian K. ; MacLeod, Kirsty J. ; Langkilde, Tracy Lee ; Kohl, Kevin D. / Gestation alters the gut microbiota of an oviparous lizard. In: FEMS microbiology ecology. 2019 ; Vol. 95, No. 7.
@article{f7e23c215d694e82ac13436a62941b90,
title = "Gestation alters the gut microbiota of an oviparous lizard",
abstract = "Mammalian pregnancy can alter the diversity, membership and structure of the maternal gut microbiota, but it is unclear whether this phenomenon occurs in vertebrates with different reproductive strategies. We conducted 16S rRNA bacterial inventories to investigate whether oviparous lizards exhibit shifts in gut microbiota similar to those observed in mammals. Using wild-caught eastern fence lizards from Alabama, USA, we collected and extracted fecal DNA from gravid and non-gravid individuals over 54 days in captivity. We predicted that, like mammals, the alpha diversity of lizard gut microbiota would decrease over gestation, and that inter-individual variation in community composition would increase. Indeed, we found that individuals in late-gestation harbored lower gut bacterial richness compared to non-gravid females. Lizard gut microbial communities of late-gestational females exhibited higher pairwise distances for both community membership and community structure compared to earlier gestation stages, indicating a higher degree of inter-individual variation as gestation progressed. Additionally, we found that the relative abundance and prevalence of the candidate phylum Melainabacteria tended to decrease over the course of gestation. While the consequences of these specific alterations are unknown, our results suggest that a general restructuring of gut microbial communities over gestation may be widespread across vertebrate reproductive strategies.",
author = "Trevelline, {Brian K.} and MacLeod, {Kirsty J.} and Langkilde, {Tracy Lee} and Kohl, {Kevin D.}",
year = "2019",
month = "7",
day = "1",
doi = "10.1093/femsec/fiz086",
language = "English (US)",
volume = "95",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "7",

}

Gestation alters the gut microbiota of an oviparous lizard. / Trevelline, Brian K.; MacLeod, Kirsty J.; Langkilde, Tracy Lee; Kohl, Kevin D.

In: FEMS microbiology ecology, Vol. 95, No. 7, 01.07.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gestation alters the gut microbiota of an oviparous lizard

AU - Trevelline, Brian K.

AU - MacLeod, Kirsty J.

AU - Langkilde, Tracy Lee

AU - Kohl, Kevin D.

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Mammalian pregnancy can alter the diversity, membership and structure of the maternal gut microbiota, but it is unclear whether this phenomenon occurs in vertebrates with different reproductive strategies. We conducted 16S rRNA bacterial inventories to investigate whether oviparous lizards exhibit shifts in gut microbiota similar to those observed in mammals. Using wild-caught eastern fence lizards from Alabama, USA, we collected and extracted fecal DNA from gravid and non-gravid individuals over 54 days in captivity. We predicted that, like mammals, the alpha diversity of lizard gut microbiota would decrease over gestation, and that inter-individual variation in community composition would increase. Indeed, we found that individuals in late-gestation harbored lower gut bacterial richness compared to non-gravid females. Lizard gut microbial communities of late-gestational females exhibited higher pairwise distances for both community membership and community structure compared to earlier gestation stages, indicating a higher degree of inter-individual variation as gestation progressed. Additionally, we found that the relative abundance and prevalence of the candidate phylum Melainabacteria tended to decrease over the course of gestation. While the consequences of these specific alterations are unknown, our results suggest that a general restructuring of gut microbial communities over gestation may be widespread across vertebrate reproductive strategies.

AB - Mammalian pregnancy can alter the diversity, membership and structure of the maternal gut microbiota, but it is unclear whether this phenomenon occurs in vertebrates with different reproductive strategies. We conducted 16S rRNA bacterial inventories to investigate whether oviparous lizards exhibit shifts in gut microbiota similar to those observed in mammals. Using wild-caught eastern fence lizards from Alabama, USA, we collected and extracted fecal DNA from gravid and non-gravid individuals over 54 days in captivity. We predicted that, like mammals, the alpha diversity of lizard gut microbiota would decrease over gestation, and that inter-individual variation in community composition would increase. Indeed, we found that individuals in late-gestation harbored lower gut bacterial richness compared to non-gravid females. Lizard gut microbial communities of late-gestational females exhibited higher pairwise distances for both community membership and community structure compared to earlier gestation stages, indicating a higher degree of inter-individual variation as gestation progressed. Additionally, we found that the relative abundance and prevalence of the candidate phylum Melainabacteria tended to decrease over the course of gestation. While the consequences of these specific alterations are unknown, our results suggest that a general restructuring of gut microbial communities over gestation may be widespread across vertebrate reproductive strategies.

UR - http://www.scopus.com/inward/record.url?scp=85069265691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85069265691&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiz086

DO - 10.1093/femsec/fiz086

M3 - Article

VL - 95

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 7

ER -