Glass-activated regeneration of volumetric muscle loss

Weitao Jia, Haoran Hu, Aize Li, Huayun Deng, Carrie L. Hogue, John C. Mauro, Changqing Zhang, Qiang Fu

Research output: Contribution to journalArticle

Abstract

Volumetric muscle loss (VML) resulting from injuries to skeletal muscles has profound consequences in healthcare. Current VML treatment based on the use of soft materials including biopolymers and decellularized extracellular matrix (dECM) is challenging due to their incapability of stimulating the formation of satellite cells (SCs), muscle stem cells, which are required for muscle regeneration. Additional stem cells and/or growth factors have to be incorporated in these constructs for improved efficacy. Here we report an approach by using bioactive glasses capable of regenerating VML without growth factors or stem cells. One silicate and two borate compositions with different degradation rates (2.4% for silicate 45S5; 5.3% and 30.4% for borate 8A3B and 13-93B3, respectively, in simulated body fluid (SBF) at 37 °C for 30 days) were used for this study. Our in vitro models demonstrate the ability of ions released from bioactive glasses in promoting angiogenesis and stimulating cells to secrete critical muscle-related growth factors. We further show the activation of SCs and the regeneration of skeletal muscles in a rat VML model. Considering these promising results, this work reveals a potentially simple and safe approach to regenerating skeletal muscle defects. Statement of significance: (1) This is the first report on an inorganic material used in skeletal muscle regeneration through in vitro and in vivo models. (2) Bioactive glass is found to activate the production of satellite cells (SCs), muscle stem cells, without the incorporation of extra stem cells or growth factors. (3) The work represents a simple, safe, low-cost yet efficient means for healing muscle defects.

Original languageEnglish (US)
Pages (from-to)306-317
Number of pages12
JournalActa Biomaterialia
Volume103
DOIs
StatePublished - Feb 2020

Fingerprint

Glass
Muscle
Regeneration
Muscles
Intercellular Signaling Peptides and Proteins
Stem cells
Silicates
Borates
Skeletal Muscle
Stem Cell Factor
Stem Cells
Bioactive glass
Muscle Cells
Skeletal Muscle Satellite Cells
Biopolymers
Satellites
Body Fluids
Extracellular Matrix
Ions
Delivery of Health Care

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Jia, Weitao ; Hu, Haoran ; Li, Aize ; Deng, Huayun ; Hogue, Carrie L. ; Mauro, John C. ; Zhang, Changqing ; Fu, Qiang. / Glass-activated regeneration of volumetric muscle loss. In: Acta Biomaterialia. 2020 ; Vol. 103. pp. 306-317.
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abstract = "Volumetric muscle loss (VML) resulting from injuries to skeletal muscles has profound consequences in healthcare. Current VML treatment based on the use of soft materials including biopolymers and decellularized extracellular matrix (dECM) is challenging due to their incapability of stimulating the formation of satellite cells (SCs), muscle stem cells, which are required for muscle regeneration. Additional stem cells and/or growth factors have to be incorporated in these constructs for improved efficacy. Here we report an approach by using bioactive glasses capable of regenerating VML without growth factors or stem cells. One silicate and two borate compositions with different degradation rates (2.4{\%} for silicate 45S5; 5.3{\%} and 30.4{\%} for borate 8A3B and 13-93B3, respectively, in simulated body fluid (SBF) at 37 °C for 30 days) were used for this study. Our in vitro models demonstrate the ability of ions released from bioactive glasses in promoting angiogenesis and stimulating cells to secrete critical muscle-related growth factors. We further show the activation of SCs and the regeneration of skeletal muscles in a rat VML model. Considering these promising results, this work reveals a potentially simple and safe approach to regenerating skeletal muscle defects. Statement of significance: (1) This is the first report on an inorganic material used in skeletal muscle regeneration through in vitro and in vivo models. (2) Bioactive glass is found to activate the production of satellite cells (SCs), muscle stem cells, without the incorporation of extra stem cells or growth factors. (3) The work represents a simple, safe, low-cost yet efficient means for healing muscle defects.",
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Jia, W, Hu, H, Li, A, Deng, H, Hogue, CL, Mauro, JC, Zhang, C & Fu, Q 2020, 'Glass-activated regeneration of volumetric muscle loss', Acta Biomaterialia, vol. 103, pp. 306-317. https://doi.org/10.1016/j.actbio.2019.12.007

Glass-activated regeneration of volumetric muscle loss. / Jia, Weitao; Hu, Haoran; Li, Aize; Deng, Huayun; Hogue, Carrie L.; Mauro, John C.; Zhang, Changqing; Fu, Qiang.

In: Acta Biomaterialia, Vol. 103, 02.2020, p. 306-317.

Research output: Contribution to journalArticle

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AU - Jia, Weitao

AU - Hu, Haoran

AU - Li, Aize

AU - Deng, Huayun

AU - Hogue, Carrie L.

AU - Mauro, John C.

AU - Zhang, Changqing

AU - Fu, Qiang

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