Global analysis of genes regulated by low temperature and photoperiod in peach bark

Carole L. Bassett, Michael E. Wisniewski, Timothy S. Artlip, John L. Norelli, Jenny Renaut, Robert E. Farrell, Jr.

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In response to environmental cues plants undergo changes in gene expression that result in the up- or down-regulation of specific genes. To identify genes in peach [Prunus persica (L.) Batsch.] trees whose transcript levels are specifically affected by low temperature (LT) or short day photoperiod (SD), we have created suppression subtractive hybridization (SSH) libraries from bark tissues sampled from trees kept at 5 °C and 25 °C under short day (SD) photoperiod or exposed to a night break (NB) interruption during the dark period of the SD cycle to simulate a long day (LD) photoperiod. Sequences expressed in forward and reverse subtractions using various subtracted combinations of temperature and photoperiod treatments were cloned, sequenced, and identified by BLAST and ClustalW analysis. Low temperature treatment resulted in the up-regulation of a number of cold-responsive and stress-related genes and suppression of genes involved in "housekeeping" functions (e.g., cell division and photosynthesis). Some stress-related genes not observed to be up-regulated under LT were increased in response to SD photoperiod treatments. Comparison of the patterns of expression as a consequence of different temperature and photoperiod treatments allowed us to determine the qualitative contribution of each treatment to the regulation of specific genes.

Original languageEnglish (US)
Pages (from-to)551-563
Number of pages13
JournalJournal of the American Society for Horticultural Science
Volume131
Issue number4
StatePublished - Jul 1 2006

Fingerprint

Photoperiod
peaches
bark
photoperiod
Temperature
Genes
temperature
genes
Up-Regulation
Housekeeping
suppression subtractive hybridization
Prunus persica
Photosynthesis
scotophase
Cell Division
Libraries
Cues
cell division
Down-Regulation
photosynthesis

All Science Journal Classification (ASJC) codes

  • Genetics
  • Horticulture

Cite this

Bassett, Carole L. ; Wisniewski, Michael E. ; Artlip, Timothy S. ; Norelli, John L. ; Renaut, Jenny ; Farrell, Jr., Robert E. / Global analysis of genes regulated by low temperature and photoperiod in peach bark. In: Journal of the American Society for Horticultural Science. 2006 ; Vol. 131, No. 4. pp. 551-563.
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Global analysis of genes regulated by low temperature and photoperiod in peach bark. / Bassett, Carole L.; Wisniewski, Michael E.; Artlip, Timothy S.; Norelli, John L.; Renaut, Jenny; Farrell, Jr., Robert E.

In: Journal of the American Society for Horticultural Science, Vol. 131, No. 4, 01.07.2006, p. 551-563.

Research output: Contribution to journalArticle

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