Global and regional cerebral blood flow in neonatal piglets undergoing pulsatile cardiopulmonary bypass with continuous perfusion at 25°C and circulatory arrest at 18°C

Akif Ündar, Michael E. DeBakey, Takafumi Masai, Shuang Qiang Yang, Harald C. Eichstaedt, Mary Claire McGarry, William K. Vaughn, Jan Goddard-Finegold, Charles D. Fraser

Research output: Contribution to journalArticle

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Abstract

To investigate the influence of hypothermic cardiopulmonary bypass (HCPB) at 25°C and circulatory arrest at 18°C on the global and regional cerebral blood flow (CBF) during pulsatile perfusion, we performed the following studies in a neonatal piglet model. Using a pediatric physiologic pulsatile pump, we subjected six piglets to deep hypothermic circulatory arrest (DHCA) and six other piglets to HCPB. The DHCA group underwent hypothermia for 25 min, DHCA for 60min, cold reperfusion for 10 min, and rewarming for 40 min. The HCPB group underwent 15 min of cooling, followed by 60 min of HCPB, 10min of cold reperfusion, and 30 min of rewarming. The following variables remained constant in both groups: pump flow (150 ml/kg/min), pump rate (150 bpm), and stroke volume (1 ml/kg). During the 60-min aortic crossclamp period, the temperature was kept at 18°C for DHCA and at 25°C for HCPB. The global and regional CBF (ml/100g/min) was assessed with radiolabeled microspheres. The CBF was 48% lower during deep hypothermia at 18°C (before DHCA) than during hypothermia at 25°C (55.2 ± 14.3 ml/100g/min vs 106.4 ± 19.7 ml/100g/min; p < 0.05). After rewarming, the global CBF was 45% lower in the DHCA group than in the HCPB group 48.3 ± 18.1 ml/100g/min vs (87 ± 35.9 ml/100g/min; p < 0.05). Fifteen minutes after the termination of CPB, the global CBF was only 25% lower in the DHCA group than in the HCPB group (42.2 ± 20.7 ml/100g/min vs 56.4 ± 25.8 ml/100g/min; p = NS). In the right and left hemispheres, cerebellum, basal ganglia, and brain stem, blood flow resembled the global CBF. In conclusion, both HCPB and DHCA significantly decrease the regional and global CBF during CPB. Unlike HCPB, DHCA has a continued negative impact on the CBF after rewarming. However, 15 min after the end of CPB, there are no significant intergroup differences in the CBF.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalPerfusion
Volume16
Issue number6
DOIs
StatePublished - Jan 1 2001

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Cerebrovascular Circulation
Deep Hypothermia Induced Circulatory Arrest
Regional Blood Flow
Cardiopulmonary Bypass
Blood
Perfusion
Rewarming
Hypothermia
Group
Pumps
Reperfusion
stroke
Pulsatile Flow
Pulsatile flow
Pediatrics
brain
Basal Ganglia
Microspheres
Stroke Volume
Cerebellum

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Safety Research
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

Ündar, Akif ; DeBakey, Michael E. ; Masai, Takafumi ; Yang, Shuang Qiang ; Eichstaedt, Harald C. ; McGarry, Mary Claire ; Vaughn, William K. ; Goddard-Finegold, Jan ; Fraser, Charles D. / Global and regional cerebral blood flow in neonatal piglets undergoing pulsatile cardiopulmonary bypass with continuous perfusion at 25°C and circulatory arrest at 18°C. In: Perfusion. 2001 ; Vol. 16, No. 6. pp. 503-510.
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abstract = "To investigate the influence of hypothermic cardiopulmonary bypass (HCPB) at 25°C and circulatory arrest at 18°C on the global and regional cerebral blood flow (CBF) during pulsatile perfusion, we performed the following studies in a neonatal piglet model. Using a pediatric physiologic pulsatile pump, we subjected six piglets to deep hypothermic circulatory arrest (DHCA) and six other piglets to HCPB. The DHCA group underwent hypothermia for 25 min, DHCA for 60min, cold reperfusion for 10 min, and rewarming for 40 min. The HCPB group underwent 15 min of cooling, followed by 60 min of HCPB, 10min of cold reperfusion, and 30 min of rewarming. The following variables remained constant in both groups: pump flow (150 ml/kg/min), pump rate (150 bpm), and stroke volume (1 ml/kg). During the 60-min aortic crossclamp period, the temperature was kept at 18°C for DHCA and at 25°C for HCPB. The global and regional CBF (ml/100g/min) was assessed with radiolabeled microspheres. The CBF was 48{\%} lower during deep hypothermia at 18°C (before DHCA) than during hypothermia at 25°C (55.2 ± 14.3 ml/100g/min vs 106.4 ± 19.7 ml/100g/min; p < 0.05). After rewarming, the global CBF was 45{\%} lower in the DHCA group than in the HCPB group 48.3 ± 18.1 ml/100g/min vs (87 ± 35.9 ml/100g/min; p < 0.05). Fifteen minutes after the termination of CPB, the global CBF was only 25{\%} lower in the DHCA group than in the HCPB group (42.2 ± 20.7 ml/100g/min vs 56.4 ± 25.8 ml/100g/min; p = NS). In the right and left hemispheres, cerebellum, basal ganglia, and brain stem, blood flow resembled the global CBF. In conclusion, both HCPB and DHCA significantly decrease the regional and global CBF during CPB. Unlike HCPB, DHCA has a continued negative impact on the CBF after rewarming. However, 15 min after the end of CPB, there are no significant intergroup differences in the CBF.",
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Global and regional cerebral blood flow in neonatal piglets undergoing pulsatile cardiopulmonary bypass with continuous perfusion at 25°C and circulatory arrest at 18°C. / Ündar, Akif; DeBakey, Michael E.; Masai, Takafumi; Yang, Shuang Qiang; Eichstaedt, Harald C.; McGarry, Mary Claire; Vaughn, William K.; Goddard-Finegold, Jan; Fraser, Charles D.

In: Perfusion, Vol. 16, No. 6, 01.01.2001, p. 503-510.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Global and regional cerebral blood flow in neonatal piglets undergoing pulsatile cardiopulmonary bypass with continuous perfusion at 25°C and circulatory arrest at 18°C

AU - Ündar, Akif

AU - DeBakey, Michael E.

AU - Masai, Takafumi

AU - Yang, Shuang Qiang

AU - Eichstaedt, Harald C.

AU - McGarry, Mary Claire

AU - Vaughn, William K.

AU - Goddard-Finegold, Jan

AU - Fraser, Charles D.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - To investigate the influence of hypothermic cardiopulmonary bypass (HCPB) at 25°C and circulatory arrest at 18°C on the global and regional cerebral blood flow (CBF) during pulsatile perfusion, we performed the following studies in a neonatal piglet model. Using a pediatric physiologic pulsatile pump, we subjected six piglets to deep hypothermic circulatory arrest (DHCA) and six other piglets to HCPB. The DHCA group underwent hypothermia for 25 min, DHCA for 60min, cold reperfusion for 10 min, and rewarming for 40 min. The HCPB group underwent 15 min of cooling, followed by 60 min of HCPB, 10min of cold reperfusion, and 30 min of rewarming. The following variables remained constant in both groups: pump flow (150 ml/kg/min), pump rate (150 bpm), and stroke volume (1 ml/kg). During the 60-min aortic crossclamp period, the temperature was kept at 18°C for DHCA and at 25°C for HCPB. The global and regional CBF (ml/100g/min) was assessed with radiolabeled microspheres. The CBF was 48% lower during deep hypothermia at 18°C (before DHCA) than during hypothermia at 25°C (55.2 ± 14.3 ml/100g/min vs 106.4 ± 19.7 ml/100g/min; p < 0.05). After rewarming, the global CBF was 45% lower in the DHCA group than in the HCPB group 48.3 ± 18.1 ml/100g/min vs (87 ± 35.9 ml/100g/min; p < 0.05). Fifteen minutes after the termination of CPB, the global CBF was only 25% lower in the DHCA group than in the HCPB group (42.2 ± 20.7 ml/100g/min vs 56.4 ± 25.8 ml/100g/min; p = NS). In the right and left hemispheres, cerebellum, basal ganglia, and brain stem, blood flow resembled the global CBF. In conclusion, both HCPB and DHCA significantly decrease the regional and global CBF during CPB. Unlike HCPB, DHCA has a continued negative impact on the CBF after rewarming. However, 15 min after the end of CPB, there are no significant intergroup differences in the CBF.

AB - To investigate the influence of hypothermic cardiopulmonary bypass (HCPB) at 25°C and circulatory arrest at 18°C on the global and regional cerebral blood flow (CBF) during pulsatile perfusion, we performed the following studies in a neonatal piglet model. Using a pediatric physiologic pulsatile pump, we subjected six piglets to deep hypothermic circulatory arrest (DHCA) and six other piglets to HCPB. The DHCA group underwent hypothermia for 25 min, DHCA for 60min, cold reperfusion for 10 min, and rewarming for 40 min. The HCPB group underwent 15 min of cooling, followed by 60 min of HCPB, 10min of cold reperfusion, and 30 min of rewarming. The following variables remained constant in both groups: pump flow (150 ml/kg/min), pump rate (150 bpm), and stroke volume (1 ml/kg). During the 60-min aortic crossclamp period, the temperature was kept at 18°C for DHCA and at 25°C for HCPB. The global and regional CBF (ml/100g/min) was assessed with radiolabeled microspheres. The CBF was 48% lower during deep hypothermia at 18°C (before DHCA) than during hypothermia at 25°C (55.2 ± 14.3 ml/100g/min vs 106.4 ± 19.7 ml/100g/min; p < 0.05). After rewarming, the global CBF was 45% lower in the DHCA group than in the HCPB group 48.3 ± 18.1 ml/100g/min vs (87 ± 35.9 ml/100g/min; p < 0.05). Fifteen minutes after the termination of CPB, the global CBF was only 25% lower in the DHCA group than in the HCPB group (42.2 ± 20.7 ml/100g/min vs 56.4 ± 25.8 ml/100g/min; p = NS). In the right and left hemispheres, cerebellum, basal ganglia, and brain stem, blood flow resembled the global CBF. In conclusion, both HCPB and DHCA significantly decrease the regional and global CBF during CPB. Unlike HCPB, DHCA has a continued negative impact on the CBF after rewarming. However, 15 min after the end of CPB, there are no significant intergroup differences in the CBF.

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