Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus

Sook Young Park, Jaeyoung Choi, Se Eun Lim, Gir Won Lee, Jongsun Park, Yang Kim, Sunghyung Kong, Se Ryun Kim, Hee Sool Rho, Junhyun Jeon, Myung Hwan Chi, Soonok Kim, Chang Hyun Khang, Seogchan Kang, Yong Hwan Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Because most efforts to understand the molecular mechanisms underpinning fungal pathogenicity have focused on studying the function and role of individual genes, relatively little is known about how transcriptional machineries globally regulate and coordinate the expression of a large group of genes involved in pathogenesis. Using quantitative real-time PCR, we analyzed the expression patterns of 206 transcription factor (TF) genes in the rice blast fungus Magnaporthe oryzae under 32 conditions, including multiple infection-related developmental stages and various abiotic stresses. The resulting data, which are publicly available via an online platform, provided new insights into how these TFs are regulated and potentially work together to control cellular responses to a diverse array of stimuli. High degrees of differential TF expression were observed under the conditions tested. More than 50% of the 206 TF genes were up-regulated during conidiation and/or in conidia. Mutations in ten conidiation-specific TF genes caused defects in conidiation. Expression patterns in planta were similar to those under oxidative stress conditions. Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice. These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes. The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.

Original languageEnglish (US)
Article numbere1003350
JournalPLoS pathogens
Volume9
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Virulence
Fungi
Transcription Factors
Genes
Oxidative Stress
Magnaporthe
Fungal Spores
Oryza
Real-Time Polymerase Chain Reaction
Mutation
Infection

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Park, Sook Young ; Choi, Jaeyoung ; Lim, Se Eun ; Lee, Gir Won ; Park, Jongsun ; Kim, Yang ; Kong, Sunghyung ; Kim, Se Ryun ; Rho, Hee Sool ; Jeon, Junhyun ; Chi, Myung Hwan ; Kim, Soonok ; Khang, Chang Hyun ; Kang, Seogchan ; Lee, Yong Hwan. / Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus. In: PLoS pathogens. 2013 ; Vol. 9, No. 6.
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abstract = "Because most efforts to understand the molecular mechanisms underpinning fungal pathogenicity have focused on studying the function and role of individual genes, relatively little is known about how transcriptional machineries globally regulate and coordinate the expression of a large group of genes involved in pathogenesis. Using quantitative real-time PCR, we analyzed the expression patterns of 206 transcription factor (TF) genes in the rice blast fungus Magnaporthe oryzae under 32 conditions, including multiple infection-related developmental stages and various abiotic stresses. The resulting data, which are publicly available via an online platform, provided new insights into how these TFs are regulated and potentially work together to control cellular responses to a diverse array of stimuli. High degrees of differential TF expression were observed under the conditions tested. More than 50{\%} of the 206 TF genes were up-regulated during conidiation and/or in conidia. Mutations in ten conidiation-specific TF genes caused defects in conidiation. Expression patterns in planta were similar to those under oxidative stress conditions. Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice. These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes. The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.",
author = "Park, {Sook Young} and Jaeyoung Choi and Lim, {Se Eun} and Lee, {Gir Won} and Jongsun Park and Yang Kim and Sunghyung Kong and Kim, {Se Ryun} and Rho, {Hee Sool} and Junhyun Jeon and Chi, {Myung Hwan} and Soonok Kim and Khang, {Chang Hyun} and Seogchan Kang and Lee, {Yong Hwan}",
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Park, SY, Choi, J, Lim, SE, Lee, GW, Park, J, Kim, Y, Kong, S, Kim, SR, Rho, HS, Jeon, J, Chi, MH, Kim, S, Khang, CH, Kang, S & Lee, YH 2013, 'Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus', PLoS pathogens, vol. 9, no. 6, e1003350. https://doi.org/10.1371/journal.ppat.1003350

Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus. / Park, Sook Young; Choi, Jaeyoung; Lim, Se Eun; Lee, Gir Won; Park, Jongsun; Kim, Yang; Kong, Sunghyung; Kim, Se Ryun; Rho, Hee Sool; Jeon, Junhyun; Chi, Myung Hwan; Kim, Soonok; Khang, Chang Hyun; Kang, Seogchan; Lee, Yong Hwan.

In: PLoS pathogens, Vol. 9, No. 6, e1003350, 01.06.2013.

Research output: Contribution to journalArticle

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AU - Park, Sook Young

AU - Choi, Jaeyoung

AU - Lim, Se Eun

AU - Lee, Gir Won

AU - Park, Jongsun

AU - Kim, Yang

AU - Kong, Sunghyung

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AU - Rho, Hee Sool

AU - Jeon, Junhyun

AU - Chi, Myung Hwan

AU - Kim, Soonok

AU - Khang, Chang Hyun

AU - Kang, Seogchan

AU - Lee, Yong Hwan

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