Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus

Sook Young Park, Jaeyoung Choi, Se Eun Lim, Gir Won Lee, Jongsun Park, Yang Kim, Sunghyung Kong, Se Ryun Kim, Hee Sool Rho, Junhyun Jeon, Myung Hwan Chi, Soonok Kim, Chang Hyun Khang, Seogchan Kang, Yong Hwan Lee

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Because most efforts to understand the molecular mechanisms underpinning fungal pathogenicity have focused on studying the function and role of individual genes, relatively little is known about how transcriptional machineries globally regulate and coordinate the expression of a large group of genes involved in pathogenesis. Using quantitative real-time PCR, we analyzed the expression patterns of 206 transcription factor (TF) genes in the rice blast fungus Magnaporthe oryzae under 32 conditions, including multiple infection-related developmental stages and various abiotic stresses. The resulting data, which are publicly available via an online platform, provided new insights into how these TFs are regulated and potentially work together to control cellular responses to a diverse array of stimuli. High degrees of differential TF expression were observed under the conditions tested. More than 50% of the 206 TF genes were up-regulated during conidiation and/or in conidia. Mutations in ten conidiation-specific TF genes caused defects in conidiation. Expression patterns in planta were similar to those under oxidative stress conditions. Mutants of in planta inducible genes not only exhibited sensitive to oxidative stress but also failed to infect rice. These experimental validations clearly demonstrated the value of TF expression patterns in predicting the function of individual TF genes. The regulatory network of TF genes revealed by this study provides a solid foundation for elucidating how M. oryzae regulates its pathogenesis, development, and stress responses.

Original languageEnglish (US)
Article numbere1003350
JournalPLoS pathogens
Volume9
Issue number6
DOIs
StatePublished - Jun 2013

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

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    Park, S. Y., Choi, J., Lim, S. E., Lee, G. W., Park, J., Kim, Y., Kong, S., Kim, S. R., Rho, H. S., Jeon, J., Chi, M. H., Kim, S., Khang, C. H., Kang, S., & Lee, Y. H. (2013). Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus. PLoS pathogens, 9(6), [e1003350]. https://doi.org/10.1371/journal.ppat.1003350