Global spread of mobile antimicrobial drug resistance determinants in human and animal escherichia coli and salmonella strains causing community-acquired infections

Remi M. Ajiboye, Owen D. Solberg, Bryan M. Lee, Eva Raphael, Chitrita Debroy, Lee W. Riley

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background. Antimicrobial drugs used in human infection treatment and animal husbandry may select for drug-resistant bacterial pathogens, which are increasingly observed worldwide. We sought to examine the extent to which identical mobile drug resistance elements are shared across common pathogens isolated from human and animal sources. Methods. We compared the distribution of one class of mobile elements-integrons and gene cassettes- among uropathogenic Escherichia coli isolates, Salmonella enterica serovar Typhimurium isolates from human diarrhea cases, and E. coli and Salmonella isolates from nonhuman sources in the United States. The sequences of the gene cassettes were also compared with those deposited in GenBank. Results. Class 1 integrons were detected in 68 (49%) of 139 uropathogenic E. coli isolates, 16 (72%) of 22 human and animal Salmonella isolates, and 120 (28%) of 436 nonhuman E. coli isolates. The most prevalent cassettes were those encoding aminoglycoside adenyltransferase A (aadA) and dihydrofolate reductase A (dfrA). Sequences of aadAl, dfrA12-orfF-aadA2, and dfrA17-aadA5 gene cassettes from 35 urinary tract infection E. coli isolates and of aadA2 and aadA12 gene cassettes from 7 Salmonella isolates were 100% identical to the corresponding cassette sequences from food animal E. coli isolates and those deposited in GenBank from a wide variety of bacteria isolated from animal hosts from distinct regions of the world. Conclusion. Common community-acquired human drug-resistant infections are caused by bacterial strains that harbor mobile drug resistance determinants of identical sequences that are found in diverse bacterial species from varied animal sources worldwide.

Original languageEnglish (US)
Pages (from-to)365-371
Number of pages7
JournalClinical Infectious Diseases
Volume49
Issue number3
DOIs
StatePublished - Aug 1 2009

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Community-Acquired Infections
Microbial Drug Resistance
Salmonella
Escherichia coli
Uropathogenic Escherichia coli
Integrons
Nucleic Acid Databases
Drug Resistance
Genes
Pharmaceutical Preparations
Animal Husbandry
Tetrahydrofolate Dehydrogenase
Salmonella enterica
Aminoglycosides
Bacterial Infections
Urinary Tract Infections
Diarrhea
Bacteria
Food
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Ajiboye, Remi M. ; Solberg, Owen D. ; Lee, Bryan M. ; Raphael, Eva ; Debroy, Chitrita ; Riley, Lee W. / Global spread of mobile antimicrobial drug resistance determinants in human and animal escherichia coli and salmonella strains causing community-acquired infections. In: Clinical Infectious Diseases. 2009 ; Vol. 49, No. 3. pp. 365-371.
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abstract = "Background. Antimicrobial drugs used in human infection treatment and animal husbandry may select for drug-resistant bacterial pathogens, which are increasingly observed worldwide. We sought to examine the extent to which identical mobile drug resistance elements are shared across common pathogens isolated from human and animal sources. Methods. We compared the distribution of one class of mobile elements-integrons and gene cassettes- among uropathogenic Escherichia coli isolates, Salmonella enterica serovar Typhimurium isolates from human diarrhea cases, and E. coli and Salmonella isolates from nonhuman sources in the United States. The sequences of the gene cassettes were also compared with those deposited in GenBank. Results. Class 1 integrons were detected in 68 (49{\%}) of 139 uropathogenic E. coli isolates, 16 (72{\%}) of 22 human and animal Salmonella isolates, and 120 (28{\%}) of 436 nonhuman E. coli isolates. The most prevalent cassettes were those encoding aminoglycoside adenyltransferase A (aadA) and dihydrofolate reductase A (dfrA). Sequences of aadAl, dfrA12-orfF-aadA2, and dfrA17-aadA5 gene cassettes from 35 urinary tract infection E. coli isolates and of aadA2 and aadA12 gene cassettes from 7 Salmonella isolates were 100{\%} identical to the corresponding cassette sequences from food animal E. coli isolates and those deposited in GenBank from a wide variety of bacteria isolated from animal hosts from distinct regions of the world. Conclusion. Common community-acquired human drug-resistant infections are caused by bacterial strains that harbor mobile drug resistance determinants of identical sequences that are found in diverse bacterial species from varied animal sources worldwide.",
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Global spread of mobile antimicrobial drug resistance determinants in human and animal escherichia coli and salmonella strains causing community-acquired infections. / Ajiboye, Remi M.; Solberg, Owen D.; Lee, Bryan M.; Raphael, Eva; Debroy, Chitrita; Riley, Lee W.

In: Clinical Infectious Diseases, Vol. 49, No. 3, 01.08.2009, p. 365-371.

Research output: Contribution to journalArticle

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T1 - Global spread of mobile antimicrobial drug resistance determinants in human and animal escherichia coli and salmonella strains causing community-acquired infections

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AU - Solberg, Owen D.

AU - Lee, Bryan M.

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AU - Debroy, Chitrita

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N2 - Background. Antimicrobial drugs used in human infection treatment and animal husbandry may select for drug-resistant bacterial pathogens, which are increasingly observed worldwide. We sought to examine the extent to which identical mobile drug resistance elements are shared across common pathogens isolated from human and animal sources. Methods. We compared the distribution of one class of mobile elements-integrons and gene cassettes- among uropathogenic Escherichia coli isolates, Salmonella enterica serovar Typhimurium isolates from human diarrhea cases, and E. coli and Salmonella isolates from nonhuman sources in the United States. The sequences of the gene cassettes were also compared with those deposited in GenBank. Results. Class 1 integrons were detected in 68 (49%) of 139 uropathogenic E. coli isolates, 16 (72%) of 22 human and animal Salmonella isolates, and 120 (28%) of 436 nonhuman E. coli isolates. The most prevalent cassettes were those encoding aminoglycoside adenyltransferase A (aadA) and dihydrofolate reductase A (dfrA). Sequences of aadAl, dfrA12-orfF-aadA2, and dfrA17-aadA5 gene cassettes from 35 urinary tract infection E. coli isolates and of aadA2 and aadA12 gene cassettes from 7 Salmonella isolates were 100% identical to the corresponding cassette sequences from food animal E. coli isolates and those deposited in GenBank from a wide variety of bacteria isolated from animal hosts from distinct regions of the world. Conclusion. Common community-acquired human drug-resistant infections are caused by bacterial strains that harbor mobile drug resistance determinants of identical sequences that are found in diverse bacterial species from varied animal sources worldwide.

AB - Background. Antimicrobial drugs used in human infection treatment and animal husbandry may select for drug-resistant bacterial pathogens, which are increasingly observed worldwide. We sought to examine the extent to which identical mobile drug resistance elements are shared across common pathogens isolated from human and animal sources. Methods. We compared the distribution of one class of mobile elements-integrons and gene cassettes- among uropathogenic Escherichia coli isolates, Salmonella enterica serovar Typhimurium isolates from human diarrhea cases, and E. coli and Salmonella isolates from nonhuman sources in the United States. The sequences of the gene cassettes were also compared with those deposited in GenBank. Results. Class 1 integrons were detected in 68 (49%) of 139 uropathogenic E. coli isolates, 16 (72%) of 22 human and animal Salmonella isolates, and 120 (28%) of 436 nonhuman E. coli isolates. The most prevalent cassettes were those encoding aminoglycoside adenyltransferase A (aadA) and dihydrofolate reductase A (dfrA). Sequences of aadAl, dfrA12-orfF-aadA2, and dfrA17-aadA5 gene cassettes from 35 urinary tract infection E. coli isolates and of aadA2 and aadA12 gene cassettes from 7 Salmonella isolates were 100% identical to the corresponding cassette sequences from food animal E. coli isolates and those deposited in GenBank from a wide variety of bacteria isolated from animal hosts from distinct regions of the world. Conclusion. Common community-acquired human drug-resistant infections are caused by bacterial strains that harbor mobile drug resistance determinants of identical sequences that are found in diverse bacterial species from varied animal sources worldwide.

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