Glucose dehydrogenase is required for normal sperm storage and utilization in female Drosophila melanogaster

Kaori Iida, Douglas R. Cavener

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Female sperm storage is a key factor for reproductive success in a variety of organisms, including Drosophila melanogaster. The spermathecae, one of the Drosophila sperm storage organs, has been suggested as a long-term storage organ because its secreted substances may enhance the quality of sperm storage. Glucose dehydrogenase (GLD) is widely expressed and secreted in the spermathecal ducts among species of the genus Drosophila. This highly conserved expression pattern suggests that this enzyme might have an important role in female fertility. Here, we examine the function of GLD in sperm storage and utilization using Gld-null mutant females. The absence of GLD reduced the amount of sperm stored in the spermathecae and led to a highly asymmetrical sperm distribution in the two spermathecal capsules of the mutant females. The storage defect was especially severe when the mutant females were crossed to a Gld-mutant male that had previously mated a few hours before the experimental cross. Under this mating condition, the mutant females stored in the spermathecae only one-third of the sperm amount of the wild-type control females. In addition, the mutant females used stored sperm at a slower rate over a longer period compared with wild-type females. Thus, our results indicate that GLD facilitates both sperm uptake and release through the spermathecal ducts.

Original languageEnglish (US)
Pages (from-to)675-681
Number of pages7
JournalJournal of Experimental Biology
Volume207
Issue number4
DOIs
StatePublished - Feb 1 2004

Fingerprint

Glucose 1-Dehydrogenase
Drosophila melanogaster
sperm
Spermatozoa
glucose
spermatozoa
mutants
storage organs
Drosophila
female fertility
reproductive success
Capsules
Fertility
defect
mutant
fertility
enzyme
uptake mechanisms

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

Cite this

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Glucose dehydrogenase is required for normal sperm storage and utilization in female Drosophila melanogaster. / Iida, Kaori; Cavener, Douglas R.

In: Journal of Experimental Biology, Vol. 207, No. 4, 01.02.2004, p. 675-681.

Research output: Contribution to journalArticle

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