Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells

Z. Spolarics, C. H. Lang, G. J. Bagby, J. J. Spitzer

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Abstract

This study assessed and compared the rate of glucose utilization, activity of the hexose-monophosphate shunt (HMS), and the oxidation of glutamine, lactate, and palmitate in Kupffer (KC), endothelial (EC), and parenchymal liver cells (PC). Cells were isolated by collagenase and pronase digestion followed by centrifugal elutriation. The freshly isolated cells were incubated in the presence of 5 mM glucose, 0.5 mM glutamine, 1 mM lactate, and 0.4 mM palmitate, and the oxidation rate of individual substrates was determined by the measurement of 14CO2 production. Glucose utilization was assessed by detritiation of [2-3H]glucose. Glucose flux through HMS was 2.6, 1.6, and 0.72 nmol·h-1·mg protein-1 in KC, EC, and PC, respectively. The oxidation rate of palmitate in PC (3.5 nmol·h-1·mg protein-1) was about twofold greater than in nonparenchymal cells. Glutamine oxidation was 6.1, 4.2, and 2.1 nmol·h-1·mg protein-1 in KC, EC, and PC, respectively. In contrast, oxidation of exogenous lactate by PC (32.1 nmol·h-1·mg protein-1) was about seven- to eightfold greater than by KC or EC. Presence of prevailing lactate concentrations did not inhibit glucose oxidation in these cells, while it attenuated glucose utilization by PC. Our data show that in the presence of a physiological substrate mixture, <20% of the ATP generated from exogenous substrates is derived from glycolysis in KC or EC. Oxidation of glutamine and palmitate are the main sources for energy in these cells. In PC, however, lactate and palmitate oxidation is responsible for ~90% of the ATP production derived from the oxidation of exogenous substrates. Our data also indicate that glutamine is an important source for the citric acid cycle intermediates in the nonparechymal cells of the liver.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume261
Issue number2 24-2
StatePublished - Jan 1 1991

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Kupffer Cells
Glutamine
Fatty Acids
Endothelial Cells
Palmitates
Liver
Glucose
Lactic Acid
Pentose Phosphate Pathway
Proteins
Adenosine Triphosphate
Pronase
Citric Acid Cycle
Collagenases
Glycolysis
Digestion

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

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title = "Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells",
abstract = "This study assessed and compared the rate of glucose utilization, activity of the hexose-monophosphate shunt (HMS), and the oxidation of glutamine, lactate, and palmitate in Kupffer (KC), endothelial (EC), and parenchymal liver cells (PC). Cells were isolated by collagenase and pronase digestion followed by centrifugal elutriation. The freshly isolated cells were incubated in the presence of 5 mM glucose, 0.5 mM glutamine, 1 mM lactate, and 0.4 mM palmitate, and the oxidation rate of individual substrates was determined by the measurement of 14CO2 production. Glucose utilization was assessed by detritiation of [2-3H]glucose. Glucose flux through HMS was 2.6, 1.6, and 0.72 nmol·h-1·mg protein-1 in KC, EC, and PC, respectively. The oxidation rate of palmitate in PC (3.5 nmol·h-1·mg protein-1) was about twofold greater than in nonparenchymal cells. Glutamine oxidation was 6.1, 4.2, and 2.1 nmol·h-1·mg protein-1 in KC, EC, and PC, respectively. In contrast, oxidation of exogenous lactate by PC (32.1 nmol·h-1·mg protein-1) was about seven- to eightfold greater than by KC or EC. Presence of prevailing lactate concentrations did not inhibit glucose oxidation in these cells, while it attenuated glucose utilization by PC. Our data show that in the presence of a physiological substrate mixture, <20{\%} of the ATP generated from exogenous substrates is derived from glycolysis in KC or EC. Oxidation of glutamine and palmitate are the main sources for energy in these cells. In PC, however, lactate and palmitate oxidation is responsible for ~90{\%} of the ATP production derived from the oxidation of exogenous substrates. Our data also indicate that glutamine is an important source for the citric acid cycle intermediates in the nonparechymal cells of the liver.",
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Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells. / Spolarics, Z.; Lang, C. H.; Bagby, G. J.; Spitzer, J. J.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 261, No. 2 24-2, 01.01.1991.

Research output: Contribution to journalArticle

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