Glutamine uptake and metabolism are coordinately regulated by ERK/MAPK during T lymphocyte activation

Erikka L. Carr, Alina Kelman, Glendon S. Wu, Ravindra Gopaul, Emilee Senkevitch, Anahit Aghvanyan, Achmed M. Turay, Kenneth A. Frauwirth

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

Activation of a naive T cell is a highly energetic event, which requires a substantial increase in nutrient metabolism. Upon stimulation, T cells increase in size, rapidly proliferate, and differentiate, all of which lead to a high demand for energetic and biosynthetic precursors. Although amino acids are the basic building blocks of protein biosynthesis and contribute to many other metabolic processes, the role of amino acid metabolism in T cell activation has not been well characterized. We have found that glutamine in particular is required for T cell function. Depletion of glutamine blocks proliferation and cytokine production, and this cannot be rescued by supplying biosynthetic precursors of glutamine. Correlating with the absolute requirement for glutamine, T cell activation induces a large increase in glutamine import, but not glutamate import, and this increase is CD28-dependent. Activation coordinately enhances expression of glutamine transporters and activities of enzymes required to allow the use of glutamine as a Krebs cycle substrate in T cells. The induction of glutamine uptake and metabolism requires ERK function, providing a link to TCR signaling. Together, these data indicate that regulation of glutamine use is an important component of T cell activation. Thus, a better understanding of glutamine sensing and use in T cells may reveal novel targets for immunomodulation.

Original languageEnglish (US)
Pages (from-to)1037-1044
Number of pages8
JournalJournal of Immunology
Volume185
Issue number2
DOIs
StatePublished - Jul 15 2010

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Lymphocyte Activation
Glutamine
T-Lymphocytes
Basic Amino Acids
Citric Acid Cycle
Immunomodulation
Protein Biosynthesis
Glutamic Acid
Cytokines
Amino Acids
Food
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Medicine(all)

Cite this

Carr, E. L., Kelman, A., Wu, G. S., Gopaul, R., Senkevitch, E., Aghvanyan, A., ... Frauwirth, K. A. (2010). Glutamine uptake and metabolism are coordinately regulated by ERK/MAPK during T lymphocyte activation. Journal of Immunology, 185(2), 1037-1044. https://doi.org/10.4049/jimmunol.0903586
Carr, Erikka L. ; Kelman, Alina ; Wu, Glendon S. ; Gopaul, Ravindra ; Senkevitch, Emilee ; Aghvanyan, Anahit ; Turay, Achmed M. ; Frauwirth, Kenneth A. / Glutamine uptake and metabolism are coordinately regulated by ERK/MAPK during T lymphocyte activation. In: Journal of Immunology. 2010 ; Vol. 185, No. 2. pp. 1037-1044.
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Carr, EL, Kelman, A, Wu, GS, Gopaul, R, Senkevitch, E, Aghvanyan, A, Turay, AM & Frauwirth, KA 2010, 'Glutamine uptake and metabolism are coordinately regulated by ERK/MAPK during T lymphocyte activation', Journal of Immunology, vol. 185, no. 2, pp. 1037-1044. https://doi.org/10.4049/jimmunol.0903586

Glutamine uptake and metabolism are coordinately regulated by ERK/MAPK during T lymphocyte activation. / Carr, Erikka L.; Kelman, Alina; Wu, Glendon S.; Gopaul, Ravindra; Senkevitch, Emilee; Aghvanyan, Anahit; Turay, Achmed M.; Frauwirth, Kenneth A.

In: Journal of Immunology, Vol. 185, No. 2, 15.07.2010, p. 1037-1044.

Research output: Contribution to journalArticle

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AU - Carr, Erikka L.

AU - Kelman, Alina

AU - Wu, Glendon S.

AU - Gopaul, Ravindra

AU - Senkevitch, Emilee

AU - Aghvanyan, Anahit

AU - Turay, Achmed M.

AU - Frauwirth, Kenneth A.

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