Glutathione disulfide variability in normal human blood

Betty Jane Mills, John Richie, Calvin A. Lang

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A prevailing opinion is that glutathione disulfide (GSSG) levels in human blood are very low, but many studies have reported variable results. Therefore, our objective was to determine valid processing conditions for GSSG measurement and apply them to normal human blood samples. Reproducibility and stability of GSSG were demonstrated in acid extracts of a single sample of fresh whole blood by repetitive measurements during a 6-h period in which the %CVs were <10. In contrast, in normal subjects tested repeatedly over several years, GSSG values ranging from <2 to 166 nmol per 1010 red blood cells were obtained and the overall %CV was 46. Lower GSSG values were obtained in hemolysates and ultrafiltrates. Thus, these results indicate that blood GSSG concentrations differ due to biological variation using optimal processing conditions

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalAnalytical Biochemistry
Volume222
Issue number1
DOIs
StatePublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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