Glutathione monoethyl ester protects against glutathione deficiencies due to aging and acetaminophen in mice

Theresa S. Chen, John Richie, Herbert T. Nagasawa, Calvin A. Lang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Our previous results indicated that glutathione (GSH) and/or cysteine (Cys) deficiency occurs in many aging tissues and also after acetaminophen (APAP) administration. The aim of this study was to investigate whether GSH monoethyl ester (GSH-OEt) can correct these deficiencies. Mice of different ages (3-31 months) through the life span were sacrificed 2 h after i.p. injection of GSH-OEt (10 mmol/kg). In separate experiments, old mice (30-31 months) received the same dose of ester 30 min before the administration of APAP (375 mg/kg) or buthionine sulfoximine (BSO, 4 mmol/kg), an inhibitor of GSH synthesis. Liver and kidney samples were analyzed for GSH and Cys by HPLC. The hepatic GSH and renal cortical GSH and Cys concentrations were about 30% lower in old mice (30-31 months) compared to mature mice (12 months). GSH-OEt corrected these aging-related decreases. APAP decreased both hepatic and renal cortical GSH and Cys concentrations in old mice, but GSH-OEt prevented these decreases. GSH-OEt also prevented the BSO-induced decreases in hepatic and renal GSH concentrations. The results demonstrated that GSH-OEt protected against GSH deficiency due to biological aging as well as APAP-induced decreases in old mice. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)127-139
Number of pages13
JournalMechanisms of Ageing and Development
Volume120
Issue number1-3
DOIs
StatePublished - Dec 1 2000

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Acetaminophen
Glutathione
Esters
Cysteine
Kidney
Liver
Buthionine Sulfoximine
S-ethyl glutathione
High Pressure Liquid Chromatography
Injections

All Science Journal Classification (ASJC) codes

  • Aging
  • Developmental Biology

Cite this

Chen, Theresa S. ; Richie, John ; Nagasawa, Herbert T. ; Lang, Calvin A. / Glutathione monoethyl ester protects against glutathione deficiencies due to aging and acetaminophen in mice. In: Mechanisms of Ageing and Development. 2000 ; Vol. 120, No. 1-3. pp. 127-139.
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Glutathione monoethyl ester protects against glutathione deficiencies due to aging and acetaminophen in mice. / Chen, Theresa S.; Richie, John; Nagasawa, Herbert T.; Lang, Calvin A.

In: Mechanisms of Ageing and Development, Vol. 120, No. 1-3, 01.12.2000, p. 127-139.

Research output: Contribution to journalArticle

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