Glycolipid analyses of light-harvesting chlorosomes from envelope protein mutants of Chlorobaculum tepidum

Yusuke Tsukatani, Tadashi Mizoguchi, Jennifer Thweatt, Marcus Tank, Donald Ashley Bryant, Hitoshi Tamiaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chlorosomes are large and efficient light-harvesting organelles in green photosynthetic bacteria, and they characteristically contain large numbers of bacteriochlorophyll c, d, or e molecules. Self-aggregated bacteriochlorophyll pigments are surrounded by a monolayer envelope membrane comprised of glycolipids and Csm proteins. Here, we analyzed glycolipid compositions of chlorosomes from the green sulfur bacterium Chlorobaculum tepidum mutants lacking one, two, or three Csm proteins by HPLC equipped with an evaporative light-scattering detector. The ratio of monogalactosyldiacylglyceride (MGDG) to rhamnosylgalactosyldiacylglyceride (RGDG) was smaller in chlorosomes from mutants lacking two or three proteins in CsmC/D/H motif family than in chlorosomes from the wild-type, whereas chlorosomes lacking CsmIJ showed relatively less RGDG than MGDG. The results suggest that the CsmC, CsmD, CsmH, and other chlorosome proteins are involved in organizing MGDG and RGDG and thereby affect the size and shape of the chlorosome.

Original languageEnglish (US)
Pages (from-to)235-241
Number of pages7
JournalPhotosynthesis research
Volume128
Issue number3
DOIs
StatePublished - Jun 1 2016

Fingerprint

Chlorobium tepidum
glycolipids
Glycolipids
Mutant Proteins
Light
mutants
Bacteria
Proteins
proteins
Chlorobi
Bacteriochlorophylls
photosynthetic bacteria
light scattering
Sulfur
Pigments
Organelles
Light scattering
detectors
organelles
Monolayers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Tsukatani, Yusuke ; Mizoguchi, Tadashi ; Thweatt, Jennifer ; Tank, Marcus ; Bryant, Donald Ashley ; Tamiaki, Hitoshi. / Glycolipid analyses of light-harvesting chlorosomes from envelope protein mutants of Chlorobaculum tepidum. In: Photosynthesis research. 2016 ; Vol. 128, No. 3. pp. 235-241.
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Glycolipid analyses of light-harvesting chlorosomes from envelope protein mutants of Chlorobaculum tepidum. / Tsukatani, Yusuke; Mizoguchi, Tadashi; Thweatt, Jennifer; Tank, Marcus; Bryant, Donald Ashley; Tamiaki, Hitoshi.

In: Photosynthesis research, Vol. 128, No. 3, 01.06.2016, p. 235-241.

Research output: Contribution to journalArticle

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AU - Bryant, Donald Ashley

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