Glycolysis is important for optimal asexual growth and formation of mature tissue cysts by Toxoplasma gondii

Anurag Shukla, Kellen L. Olszewski, Manuel Llinas, Leah M. Rommereim, Barbara A. Fox, David J. Bzik, Dong Xia, Jonathan Wastling, Daniel Beiting, David S. Roos, Dhanasekaran Shanmugam

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Toxoplasma gondii can grow and replicate using either glucose or glutamine as the major carbon source. Here, we have studied the essentiality of glycolysis in the tachyzoite and bradyzoite stages of T. gondii, using transgenic parasites that lack a functional hexokinase gene (Δhk) in RH (Type-1) and Prugniaud (Type-II) strain parasites. Tachyzoite stage Δhk parasites exhibit a fitness defect similar to that reported previously for the major glucose transporter mutant, and remain virulent in mice. However, although Prugniaud strain Δhk tachyzoites were capable of transforming into bradyzoites in vitro, they were severely compromised in their ability to make mature bradyzoite cysts in the brain tissue of mice. Isotopic labelling studies reveal that glucose-deprived tacyzoites utilise glutamine to replenish glycolytic and pentose phosphate pathway intermediates via gluconeogenesis. Interestingly, while glutamine-deprived intracellular Δhk tachyzoites continued to replicate, extracellular parasites were unable to efficiently invade host cells. Further, studies on mutant tachyzoites lacking a functional phosphoenolpyruvate carboxykinase (Δpepck1) revealed that glutaminolysis is the sole source of gluconeogenic flux in glucose-deprived parasites. In addition, glutaminolysis is essential for sustaining oxidative phosphorylation in Δhk parasites, while wild type (wt) and Δpepck1 parasites can obtain ATP from either glycolysis or oxidative phosphorylation. This study provides insights into the role of nutrient metabolism during asexual propagation and development of T. gondii, and validates the versatile nature of central carbon and energy metabolism in this parasite.

Original languageEnglish (US)
Pages (from-to)955-968
Number of pages14
JournalInternational Journal for Parasitology
Volume48
Issue number12
DOIs
StatePublished - Oct 1 2018

Fingerprint

Toxoplasma
Glycolysis
Cysts
Parasites
Growth
Glutamine
Oxidative Phosphorylation
Glucose
Carbon
Pentose Phosphate Pathway
Phosphoenolpyruvate
Hexokinase
Gluconeogenesis
Facilitative Glucose Transport Proteins
Energy Metabolism
Adenosine Triphosphate
Food
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Shukla, Anurag ; Olszewski, Kellen L. ; Llinas, Manuel ; Rommereim, Leah M. ; Fox, Barbara A. ; Bzik, David J. ; Xia, Dong ; Wastling, Jonathan ; Beiting, Daniel ; Roos, David S. ; Shanmugam, Dhanasekaran. / Glycolysis is important for optimal asexual growth and formation of mature tissue cysts by Toxoplasma gondii. In: International Journal for Parasitology. 2018 ; Vol. 48, No. 12. pp. 955-968.
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abstract = "Toxoplasma gondii can grow and replicate using either glucose or glutamine as the major carbon source. Here, we have studied the essentiality of glycolysis in the tachyzoite and bradyzoite stages of T. gondii, using transgenic parasites that lack a functional hexokinase gene (Δhk) in RH (Type-1) and Prugniaud (Type-II) strain parasites. Tachyzoite stage Δhk parasites exhibit a fitness defect similar to that reported previously for the major glucose transporter mutant, and remain virulent in mice. However, although Prugniaud strain Δhk tachyzoites were capable of transforming into bradyzoites in vitro, they were severely compromised in their ability to make mature bradyzoite cysts in the brain tissue of mice. Isotopic labelling studies reveal that glucose-deprived tacyzoites utilise glutamine to replenish glycolytic and pentose phosphate pathway intermediates via gluconeogenesis. Interestingly, while glutamine-deprived intracellular Δhk tachyzoites continued to replicate, extracellular parasites were unable to efficiently invade host cells. Further, studies on mutant tachyzoites lacking a functional phosphoenolpyruvate carboxykinase (Δpepck1) revealed that glutaminolysis is the sole source of gluconeogenic flux in glucose-deprived parasites. In addition, glutaminolysis is essential for sustaining oxidative phosphorylation in Δhk parasites, while wild type (wt) and Δpepck1 parasites can obtain ATP from either glycolysis or oxidative phosphorylation. This study provides insights into the role of nutrient metabolism during asexual propagation and development of T. gondii, and validates the versatile nature of central carbon and energy metabolism in this parasite.",
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Shukla, A, Olszewski, KL, Llinas, M, Rommereim, LM, Fox, BA, Bzik, DJ, Xia, D, Wastling, J, Beiting, D, Roos, DS & Shanmugam, D 2018, 'Glycolysis is important for optimal asexual growth and formation of mature tissue cysts by Toxoplasma gondii', International Journal for Parasitology, vol. 48, no. 12, pp. 955-968. https://doi.org/10.1016/j.ijpara.2018.05.013

Glycolysis is important for optimal asexual growth and formation of mature tissue cysts by Toxoplasma gondii. / Shukla, Anurag; Olszewski, Kellen L.; Llinas, Manuel; Rommereim, Leah M.; Fox, Barbara A.; Bzik, David J.; Xia, Dong; Wastling, Jonathan; Beiting, Daniel; Roos, David S.; Shanmugam, Dhanasekaran.

In: International Journal for Parasitology, Vol. 48, No. 12, 01.10.2018, p. 955-968.

Research output: Contribution to journalArticle

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T1 - Glycolysis is important for optimal asexual growth and formation of mature tissue cysts by Toxoplasma gondii

AU - Shukla, Anurag

AU - Olszewski, Kellen L.

AU - Llinas, Manuel

AU - Rommereim, Leah M.

AU - Fox, Barbara A.

AU - Bzik, David J.

AU - Xia, Dong

AU - Wastling, Jonathan

AU - Beiting, Daniel

AU - Roos, David S.

AU - Shanmugam, Dhanasekaran

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Toxoplasma gondii can grow and replicate using either glucose or glutamine as the major carbon source. Here, we have studied the essentiality of glycolysis in the tachyzoite and bradyzoite stages of T. gondii, using transgenic parasites that lack a functional hexokinase gene (Δhk) in RH (Type-1) and Prugniaud (Type-II) strain parasites. Tachyzoite stage Δhk parasites exhibit a fitness defect similar to that reported previously for the major glucose transporter mutant, and remain virulent in mice. However, although Prugniaud strain Δhk tachyzoites were capable of transforming into bradyzoites in vitro, they were severely compromised in their ability to make mature bradyzoite cysts in the brain tissue of mice. Isotopic labelling studies reveal that glucose-deprived tacyzoites utilise glutamine to replenish glycolytic and pentose phosphate pathway intermediates via gluconeogenesis. Interestingly, while glutamine-deprived intracellular Δhk tachyzoites continued to replicate, extracellular parasites were unable to efficiently invade host cells. Further, studies on mutant tachyzoites lacking a functional phosphoenolpyruvate carboxykinase (Δpepck1) revealed that glutaminolysis is the sole source of gluconeogenic flux in glucose-deprived parasites. In addition, glutaminolysis is essential for sustaining oxidative phosphorylation in Δhk parasites, while wild type (wt) and Δpepck1 parasites can obtain ATP from either glycolysis or oxidative phosphorylation. This study provides insights into the role of nutrient metabolism during asexual propagation and development of T. gondii, and validates the versatile nature of central carbon and energy metabolism in this parasite.

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