GODZ-mediated palmitoylation of GABAA receptors is required for normal assembly and function of GABAergic inhibitory synapses

Cheng Fang, Lunbin Deng, Cheryl A. Keller Capone, Masaki Fukata, Yuko Fukata, Gong Chen, Bernhard Luscher

Research output: Contribution to journalArticle

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Abstract

Golgi-specific DHHC (Asp-His-His-Cys) zinc finger protein (GODZ) is a DHHC family palmitoyl acyltransferase that is implicated in palmitoylation and regulated trafficking of diverse substrates that function either at inhibitory or excitatory synapses. Of particular interest is the γ2 subunit of GABAA receptors, which is required for targeting these receptors to inhibitory synapses. Here, we report that GODZ and, to a lesser extent, its close paralog sertoli cell gene with a zinc finger domain-β (SERZ-β) are the main members of the DHHC family of enzymes that are able to palmitoylate the γ2 subunit in heterologous cells. Yeast two-hybrid and colocalization assays in human embryonic kidney 293T (HEK293T) cells indicate that GODZ and SERZ-β show indistinguishable palmitoylation-dependent interaction with the γ2 subunit. After coexpression in HEK293T cells, they form homomultimers and heteromultimers, as shown by coimmunoprecipitation and in vivo cross-linking experiments. Analyses in neurons transfected with dominant-negative GODZ (GODZC157S) or plasmid-based GODZ-specific RNAi indicate that GODZ is required for normal accumulation of GABAA receptors at synapses, for normal whole-cell and synaptic GABAergic inhibitory function and, indirectly, for GABAergic innervation. Unexpectedly, GODZ was found to be dispensable for normal postsynaptic AMPA receptor-mediated glutamatergic transmission. We conclude that GODZ-mediated palmitoylation of GABAA receptors and possibly other substrates contributes selectively to the formation and normal function of GABAergic inhibitory synapses.

Original languageEnglish (US)
Pages (from-to)12758-12768
Number of pages11
JournalJournal of Neuroscience
Volume26
Issue number49
DOIs
StatePublished - Dec 6 2006

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Lipoylation
GABA-A Receptors
Synapses
HEK293 Cells
Zinc Fingers
Kidney
Acyltransferases
Two-Hybrid System Techniques
AMPA Receptors
Sertoli Cells
RNA Interference
Plasmids
Neurons
Enzymes
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "GODZ-mediated palmitoylation of GABAA receptors is required for normal assembly and function of GABAergic inhibitory synapses",
abstract = "Golgi-specific DHHC (Asp-His-His-Cys) zinc finger protein (GODZ) is a DHHC family palmitoyl acyltransferase that is implicated in palmitoylation and regulated trafficking of diverse substrates that function either at inhibitory or excitatory synapses. Of particular interest is the γ2 subunit of GABAA receptors, which is required for targeting these receptors to inhibitory synapses. Here, we report that GODZ and, to a lesser extent, its close paralog sertoli cell gene with a zinc finger domain-β (SERZ-β) are the main members of the DHHC family of enzymes that are able to palmitoylate the γ2 subunit in heterologous cells. Yeast two-hybrid and colocalization assays in human embryonic kidney 293T (HEK293T) cells indicate that GODZ and SERZ-β show indistinguishable palmitoylation-dependent interaction with the γ2 subunit. After coexpression in HEK293T cells, they form homomultimers and heteromultimers, as shown by coimmunoprecipitation and in vivo cross-linking experiments. Analyses in neurons transfected with dominant-negative GODZ (GODZC157S) or plasmid-based GODZ-specific RNAi indicate that GODZ is required for normal accumulation of GABAA receptors at synapses, for normal whole-cell and synaptic GABAergic inhibitory function and, indirectly, for GABAergic innervation. Unexpectedly, GODZ was found to be dispensable for normal postsynaptic AMPA receptor-mediated glutamatergic transmission. We conclude that GODZ-mediated palmitoylation of GABAA receptors and possibly other substrates contributes selectively to the formation and normal function of GABAergic inhibitory synapses.",
author = "Cheng Fang and Lunbin Deng and {Keller Capone}, {Cheryl A.} and Masaki Fukata and Yuko Fukata and Gong Chen and Bernhard Luscher",
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GODZ-mediated palmitoylation of GABAA receptors is required for normal assembly and function of GABAergic inhibitory synapses. / Fang, Cheng; Deng, Lunbin; Keller Capone, Cheryl A.; Fukata, Masaki; Fukata, Yuko; Chen, Gong; Luscher, Bernhard.

In: Journal of Neuroscience, Vol. 26, No. 49, 06.12.2006, p. 12758-12768.

Research output: Contribution to journalArticle

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T1 - GODZ-mediated palmitoylation of GABAA receptors is required for normal assembly and function of GABAergic inhibitory synapses

AU - Fang, Cheng

AU - Deng, Lunbin

AU - Keller Capone, Cheryl A.

AU - Fukata, Masaki

AU - Fukata, Yuko

AU - Chen, Gong

AU - Luscher, Bernhard

PY - 2006/12/6

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AB - Golgi-specific DHHC (Asp-His-His-Cys) zinc finger protein (GODZ) is a DHHC family palmitoyl acyltransferase that is implicated in palmitoylation and regulated trafficking of diverse substrates that function either at inhibitory or excitatory synapses. Of particular interest is the γ2 subunit of GABAA receptors, which is required for targeting these receptors to inhibitory synapses. Here, we report that GODZ and, to a lesser extent, its close paralog sertoli cell gene with a zinc finger domain-β (SERZ-β) are the main members of the DHHC family of enzymes that are able to palmitoylate the γ2 subunit in heterologous cells. Yeast two-hybrid and colocalization assays in human embryonic kidney 293T (HEK293T) cells indicate that GODZ and SERZ-β show indistinguishable palmitoylation-dependent interaction with the γ2 subunit. After coexpression in HEK293T cells, they form homomultimers and heteromultimers, as shown by coimmunoprecipitation and in vivo cross-linking experiments. Analyses in neurons transfected with dominant-negative GODZ (GODZC157S) or plasmid-based GODZ-specific RNAi indicate that GODZ is required for normal accumulation of GABAA receptors at synapses, for normal whole-cell and synaptic GABAergic inhibitory function and, indirectly, for GABAergic innervation. Unexpectedly, GODZ was found to be dispensable for normal postsynaptic AMPA receptor-mediated glutamatergic transmission. We conclude that GODZ-mediated palmitoylation of GABAA receptors and possibly other substrates contributes selectively to the formation and normal function of GABAergic inhibitory synapses.

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