Gradients of lipid storage, photosynthesis and plastid differentiation in developing soybean seeds

Ljudmilla Borisjuk, Thuy Ha Nguyen, Thomas Neuberger, Twan Rutten, Henning Tschiersch, Bernhard Claus, Ivo Feussner, Andrew G. Webb, Peter Jakob, Hans Weber, Ulrich Wobus, Hardy Rolletschek

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

• This study establishes a topographical framework for functional investigations on the regulation of lipid biosynthesis and its interaction with embryo photosynthesis in developing soybean seed. • Structural observations, combined with molecular and functional parameters, revealed the gradual transformation of chloroplasts into storage organelles, starting from inner regions going outwards. This is evidenced by electron microscopy, confocal laser scanning microscopy, in situ hybridization and histochemical/biochemical data. • As a consequence of plastid differentiation, photosynthesis becomes distributed along a gradient within the developing embryo. Electron transport rate, effective quantum yield and O2 production rate are maximal in the embryo periphery, as documented by imaging pulse-amplitude-modulated fluorescence and O2 release via microsensors. The gradual loss of photosynthetic capacity was accompanied by a similarly gradual accumulation of starch and lipids. Noninvasive nuclear magnetic resonance spectroscopy of mature seeds revealed steep gradients in lipid deposition, with the highest concentrations in inner regions. • The inverse relationship between photosynthesis and lipid biosynthesis argues against a direct metabolic involvement of photosynthesis in lipid biosynthesis during the late storage stage, but points to a role for photosynthetic oxygen release. This hypothesis is verified in a companion paper.

Original languageEnglish (US)
Pages (from-to)761-776
Number of pages16
JournalNew Phytologist
Volume167
Issue number3
DOIs
StatePublished - Sep 1 2005

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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