Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato

F. Di Gioia, A. Aprile, E. Sabella, P. Santamaria, A. Pardossi, A. Miceli, L. De Bellis, E. Nutricati

Research output: Contribution to journalArticle

Abstract

Boron (B) is essential for plant growth, however its excess in soil and/or in irrigation water can severely compromise plant growth and yield. The goal of this work was to determine whether grafting onto ‘Arnold’, a commercial interspecific hybrid (Solanum lycopersicum × S. habrochaites) rootstock, which in a previous study was found to be tolerant to salt stress, could improve tomato (S. lycopersicum L. ‘Ikram’) tolerance to excess B, and whether this effect is associated with an exclusion mechanism. Non-grafted, self-grafted and grafted plants were hydroponically grown in a greenhouse with B concentration in the nutrient solution of 0.27 (control), 5, 10 and 15 mg·l−1. A transcription analysis was carried out on SlNIP5 and SlBOR1 genes, which encode putative B transporters. Grafting ‘Ikram’ onto ‘Arnold’ rootstock reduced B concentration in leaf tissue of plants exposed to B concentrations of 10–15 mg·l−1. At high B levels, SlNIP5 was down-regulated in all grafting combinations, while SlBOR1 was down-regulated only in the roots of plants grafted onto ‘Arnold’. We conclude that grafting the susceptible tomato cultivar ‘Ikram’ onto the commercial rootstock ‘Arnold’ improved tolerance to excess B by reducing expression of genes encoding for B transporters at the root level, thus partially reducing the root uptake of B and its accumulation in the shoot.

Original languageEnglish (US)
Pages (from-to)728-735
Number of pages8
JournalPlant Biology
Volume19
Issue number5
DOIs
StatePublished - Sep 2017

Fingerprint

grafting (plants)
boron
transporters
rootstock
tomatoes
rootstocks
gene
genes
plant growth
tolerance
Solanum
irrigation water
nutrient solutions
salt stress
plant tissues
transcription (genetics)
cultivar
greenhouses
uptake mechanisms
gene expression

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Di Gioia, F., Aprile, A., Sabella, E., Santamaria, P., Pardossi, A., Miceli, A., ... Nutricati, E. (2017). Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato. Plant Biology, 19(5), 728-735. https://doi.org/10.1111/plb.12589
Di Gioia, F. ; Aprile, A. ; Sabella, E. ; Santamaria, P. ; Pardossi, A. ; Miceli, A. ; De Bellis, L. ; Nutricati, E. / Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato. In: Plant Biology. 2017 ; Vol. 19, No. 5. pp. 728-735.
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Di Gioia, F, Aprile, A, Sabella, E, Santamaria, P, Pardossi, A, Miceli, A, De Bellis, L & Nutricati, E 2017, 'Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato', Plant Biology, vol. 19, no. 5, pp. 728-735. https://doi.org/10.1111/plb.12589

Grafting response to excess boron and expression analysis of genes coding boron transporters in tomato. / Di Gioia, F.; Aprile, A.; Sabella, E.; Santamaria, P.; Pardossi, A.; Miceli, A.; De Bellis, L.; Nutricati, E.

In: Plant Biology, Vol. 19, No. 5, 09.2017, p. 728-735.

Research output: Contribution to journalArticle

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