Greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease

Andrew W. Gardner, Donald E. Parker, Polly S. Montgomery, Danuta Sosnowska, Ana I. Casanegra, Zoltan Ungvari, Anna Csiszar, William E. Sonntag

Research output: Contribution to journalArticle

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Abstract

We compared apoptosis, cellular oxidative stress, and inflammation of cultured endothelial cells treated with sera from 156 subjects with peripheral artery disease (PAD) and 16 healthy control subjects. Furthermore, we compared circulating inflammatory, antioxidant capacity, and vascular biomarkers between the two groups. The PAD group had a 164% higher value for endothelial cell apoptosis (P < 0.001) and a 62% higher value for endothelial cellular reactive oxygen species production (P < 0.001) than the control group. Furthermore, the PAD group had lower systemic antioxidant capacity measured by hydroxyl radical antioxidant capacity activity (P < 0.001), higher inflammatory and vascular measures of high-sensitivity C-reactive protein (P < 0.001), interleukin-8 (P < 0.001), serum amyloid A (P < 0.001), vascular cell adhesion molecule-1 (P < 0.001), adiponectin (P < 0.001), apolipoprotein B (P = 0.013), apolipoprotein CIII (P = 0.035), lower vascular endothelial growth factor-A (P < 0.001), and hepatocyte growth factor (P < 0.001) than the control group. Subjects with PAD have greater endothelial apoptosis and oxidative stress than control subjects with low burden of comorbid conditions and cardiovascular risk factors. Furthermore, subjects with PAD have lower systemic antioxidant capacity and angiogenic measures and higher circulating inflammatory parameters.

Original languageEnglish (US)
Article number160534
JournalInternational Journal of Vascular Medicine
Volume2014
DOIs
StatePublished - 2014

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Peripheral Arterial Disease
Oxidative Stress
Apoptosis
Antioxidants
Blood Vessels
Endothelial Cells
Apolipoprotein C-III
Serum Amyloid A Protein
Control Groups
Hepatocyte Growth Factor
Vascular Cell Adhesion Molecule-1
Adiponectin
Apolipoproteins B
Interleukin-8
Hydroxyl Radical
C-Reactive Protein
Vascular Endothelial Growth Factor A
Cultured Cells
Reactive Oxygen Species
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Gardner, A. W., Parker, D. E., Montgomery, P. S., Sosnowska, D., Casanegra, A. I., Ungvari, Z., ... Sonntag, W. E. (2014). Greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease. International Journal of Vascular Medicine, 2014, [160534]. https://doi.org/10.1155/2014/160534
Gardner, Andrew W. ; Parker, Donald E. ; Montgomery, Polly S. ; Sosnowska, Danuta ; Casanegra, Ana I. ; Ungvari, Zoltan ; Csiszar, Anna ; Sonntag, William E. / Greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease. In: International Journal of Vascular Medicine. 2014 ; Vol. 2014.
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abstract = "We compared apoptosis, cellular oxidative stress, and inflammation of cultured endothelial cells treated with sera from 156 subjects with peripheral artery disease (PAD) and 16 healthy control subjects. Furthermore, we compared circulating inflammatory, antioxidant capacity, and vascular biomarkers between the two groups. The PAD group had a 164{\%} higher value for endothelial cell apoptosis (P < 0.001) and a 62{\%} higher value for endothelial cellular reactive oxygen species production (P < 0.001) than the control group. Furthermore, the PAD group had lower systemic antioxidant capacity measured by hydroxyl radical antioxidant capacity activity (P < 0.001), higher inflammatory and vascular measures of high-sensitivity C-reactive protein (P < 0.001), interleukin-8 (P < 0.001), serum amyloid A (P < 0.001), vascular cell adhesion molecule-1 (P < 0.001), adiponectin (P < 0.001), apolipoprotein B (P = 0.013), apolipoprotein CIII (P = 0.035), lower vascular endothelial growth factor-A (P < 0.001), and hepatocyte growth factor (P < 0.001) than the control group. Subjects with PAD have greater endothelial apoptosis and oxidative stress than control subjects with low burden of comorbid conditions and cardiovascular risk factors. Furthermore, subjects with PAD have lower systemic antioxidant capacity and angiogenic measures and higher circulating inflammatory parameters.",
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Gardner, AW, Parker, DE, Montgomery, PS, Sosnowska, D, Casanegra, AI, Ungvari, Z, Csiszar, A & Sonntag, WE 2014, 'Greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease', International Journal of Vascular Medicine, vol. 2014, 160534. https://doi.org/10.1155/2014/160534

Greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease. / Gardner, Andrew W.; Parker, Donald E.; Montgomery, Polly S.; Sosnowska, Danuta; Casanegra, Ana I.; Ungvari, Zoltan; Csiszar, Anna; Sonntag, William E.

In: International Journal of Vascular Medicine, Vol. 2014, 160534, 2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease

AU - Gardner, Andrew W.

AU - Parker, Donald E.

AU - Montgomery, Polly S.

AU - Sosnowska, Danuta

AU - Casanegra, Ana I.

AU - Ungvari, Zoltan

AU - Csiszar, Anna

AU - Sonntag, William E.

PY - 2014

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AB - We compared apoptosis, cellular oxidative stress, and inflammation of cultured endothelial cells treated with sera from 156 subjects with peripheral artery disease (PAD) and 16 healthy control subjects. Furthermore, we compared circulating inflammatory, antioxidant capacity, and vascular biomarkers between the two groups. The PAD group had a 164% higher value for endothelial cell apoptosis (P < 0.001) and a 62% higher value for endothelial cellular reactive oxygen species production (P < 0.001) than the control group. Furthermore, the PAD group had lower systemic antioxidant capacity measured by hydroxyl radical antioxidant capacity activity (P < 0.001), higher inflammatory and vascular measures of high-sensitivity C-reactive protein (P < 0.001), interleukin-8 (P < 0.001), serum amyloid A (P < 0.001), vascular cell adhesion molecule-1 (P < 0.001), adiponectin (P < 0.001), apolipoprotein B (P = 0.013), apolipoprotein CIII (P = 0.035), lower vascular endothelial growth factor-A (P < 0.001), and hepatocyte growth factor (P < 0.001) than the control group. Subjects with PAD have greater endothelial apoptosis and oxidative stress than control subjects with low burden of comorbid conditions and cardiovascular risk factors. Furthermore, subjects with PAD have lower systemic antioxidant capacity and angiogenic measures and higher circulating inflammatory parameters.

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