Green fluorescent protein purification by organic extraction

Alexander V. Yakhnin, Leonid M. Vinokurov, Alexey K. Surin, Yuli B. Alakhov

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Green fluorescent protein (GFP) is widely used as an excellent reporter molecule in biochemistry and cell biology. Some biochemical and immunological assays require high-purity GFP. However, the majority of current procedures for GFP purification include multiple time-consuming chromatography steps with a low yield of the desired product or require tag-containing proteins. An alternative method is described for the GFP purification without affinity extensions using organic extraction yielding a highly homogeneous protein indistinguishable in spectroscopic properties from that purified by previous methods.

Original languageEnglish (US)
Pages (from-to)382-386
Number of pages5
JournalProtein Expression and Purification
Volume14
Issue number3
DOIs
StatePublished - Dec 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology

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