Growth and pigment production by hairy root cultures of Beta vulgaris L. in a bubble column reactor

Usha Mukundan, Edgard B. Carvalho, Wayne R. Curtis

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Beet hairy root cultures established from red and yellow varieties were grown in a 2 L bubble column reactor. The yellow clone showed profuse root hairs and a predominance of betaxanthin pigment while the red clone showed fewer root hairs and both betaxanthin and betacyanin pigments. The cultures displayed different ionic and sugar yields: 2.1 mg dry wt/mS.mL and 0.361 g dry wt/g sugar for the yellow clone and 2.3 mg dry wt/mS.mL and 0.375 g dry wt/g sugar for the red one. Both cultures grew at the same specific growth rate of 0.22 d-1 in the bubble column, as compared to 0.32 d-1 in shake flasks, indicating mass transfer limitations for growth in reactors.

Original languageEnglish (US)
Pages (from-to)469-474
Number of pages6
JournalBiotechnology Letters
Volume20
Issue number5
DOIs
StatePublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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