Growth at low temperature causes nitrogen limitation in the cyanobacterium Synechococcus sp. PCC 7002

Toshio Sakamoto, Donald Ashley Bryant

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The coloration of cells of the cyanobacterium Synechococcus sp. PCC 7002 changed from normal blue-green to yellow-green when cells were grown at 15°C in a medium containing nitrate as the sole nitrogen source. This change of coloration was similar to a general response to nutrient deprivation (chlorosis). For the chlorotic cells at 15°C, the total amounts of phycobiliproteins and chlorophyll a decreased, high levels of glycogen accumulated, and growth was arithmetic rather than exponential. These changes in composition and growth occurred in cells grown at low (50 μEm-2 s-1) as well as high (250 μE m-2 s-1) light intensity. After a temperature shift-up to 38°C, chlorotic cells rapidly regained their normal blue-green coloration and normal exponential growth rate within 7 h. When cells were grown at 15°C in a medium containing urea as the reduced nitrogen source, cells grew exponentially and the symptoms of chlorosis were not observed. The decrease in photosynthetic oxygen evolution activity at low temperature was much smaller than the decrease in growth rate for cells grown on nitrate as the nitrogen source. These studies demonstrate that low-temperature-induced chlorosis of Synechococcus sp. PCC 7002 is caused by nitrogen limitation and is not the result of limited photosynthetic activity or photodamage to the photosynthetic apparatus, and that nitrogen assimilation is an important aspect of the low-temperature physiology of cyanobacteria.

Original languageEnglish (US)
Pages (from-to)10-19
Number of pages10
JournalArchives of Microbiology
Volume169
Issue number1
DOIs
StatePublished - Dec 1 1997

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Synechococcus
Cyanobacteria
Nitrogen
Temperature
Growth
Hypochromic Anemia
Nitrates
Phycobiliproteins
Cells
Physiology
Glycogen
Nutrients
Urea
Oxygen
Chemical analysis
Light
Food

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

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abstract = "The coloration of cells of the cyanobacterium Synechococcus sp. PCC 7002 changed from normal blue-green to yellow-green when cells were grown at 15°C in a medium containing nitrate as the sole nitrogen source. This change of coloration was similar to a general response to nutrient deprivation (chlorosis). For the chlorotic cells at 15°C, the total amounts of phycobiliproteins and chlorophyll a decreased, high levels of glycogen accumulated, and growth was arithmetic rather than exponential. These changes in composition and growth occurred in cells grown at low (50 μEm-2 s-1) as well as high (250 μE m-2 s-1) light intensity. After a temperature shift-up to 38°C, chlorotic cells rapidly regained their normal blue-green coloration and normal exponential growth rate within 7 h. When cells were grown at 15°C in a medium containing urea as the reduced nitrogen source, cells grew exponentially and the symptoms of chlorosis were not observed. The decrease in photosynthetic oxygen evolution activity at low temperature was much smaller than the decrease in growth rate for cells grown on nitrate as the nitrogen source. These studies demonstrate that low-temperature-induced chlorosis of Synechococcus sp. PCC 7002 is caused by nitrogen limitation and is not the result of limited photosynthetic activity or photodamage to the photosynthetic apparatus, and that nitrogen assimilation is an important aspect of the low-temperature physiology of cyanobacteria.",
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Growth at low temperature causes nitrogen limitation in the cyanobacterium Synechococcus sp. PCC 7002. / Sakamoto, Toshio; Bryant, Donald Ashley.

In: Archives of Microbiology, Vol. 169, No. 1, 01.12.1997, p. 10-19.

Research output: Contribution to journalArticle

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