Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize

Jie Wang, Mingyu Yang, Yuanyuan Song, Flor E. Acevedo, Kelli Hoover, Rensen Zeng, Gary Felton

Research output: Contribution to journalArticle

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Abstract

Insect-associated microbes can contribute to the physiological and ecological functions of insects. Despite a few examples in beetles and piercing-sucking insects, the varied mechanisms of how insect-associated bacteria mediate plant-insect interactions are still not fully understood. The polyphagous herbivore Helicoverpa zea is a major agricultural pest that harbors certain microbes in their digestive systems. Enterobacter ludwigii is one of the gut-associated bacteria identified from field-collected caterpillars, and it has been shown to indirectly induce defenses in the dicot plant tomato by triggering the biosynthesis of salivary elicitors, but there are no clear mechanisms to show how gut microbes alter these salivary cues and how a different host plant responds to these inducible elicitors. Here, we conducted a series of assays to determine whether infection with E. ludwigii affects H. zea larval growth, immunity, and salivary responses and thus influences induced defenses of maize to herbivory. Inoculating lab-reared caterpillars with E. ludwigii, did not significantly affect the growth of caterpillars, but two immunity-related genes glucose oxidase (GOX) and lysozyme (LYZ) were more highly expressed in both salivary glands and midguts compared with MgCl2 solution-treated caterpillars. Oral elicitors were evaluated for their role in triggering maize-specific defense responses. Our results show that saliva and its main component protein glucose oxidase (GOX) from E. ludwigii-inoculated caterpillars played a role in inducing maize anti-herbivore responses. These findings provide a novel concept that introducing bacteria to an herbivore may be an important approach to pest control through alteration of insect immune responses and thus indirect induction of plant resistance.

Original languageEnglish (US)
Pages (from-to)690-699
Number of pages10
JournalJournal of Chemical Ecology
Volume44
Issue number7-8
DOIs
StatePublished - Aug 1 2018

Fingerprint

plant defense
Helicoverpa zea
caterpillar
Zea mays
Insects
insect larvae
Bacteria
Glucose Oxidase
Herbivory
digestive system
maize
insect
herbivores
insects
bacterium
corn
bacteria
Digestive system
glucose oxidase
herbivore

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Wang, Jie ; Yang, Mingyu ; Song, Yuanyuan ; Acevedo, Flor E. ; Hoover, Kelli ; Zeng, Rensen ; Felton, Gary. / Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize. In: Journal of Chemical Ecology. 2018 ; Vol. 44, No. 7-8. pp. 690-699.
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Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize. / Wang, Jie; Yang, Mingyu; Song, Yuanyuan; Acevedo, Flor E.; Hoover, Kelli; Zeng, Rensen; Felton, Gary.

In: Journal of Chemical Ecology, Vol. 44, No. 7-8, 01.08.2018, p. 690-699.

Research output: Contribution to journalArticle

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