Gut microbes contribute to nitrogen provisioning in a wood-feeding cerambycid

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Xylophagous insects often thrive on nutritionally suboptimal diets through symbiotic associations with microbes that supplement their nutritional requirements, particularly nitrogen. The wood-feeding cerambycid Anoplophora glabripennis (Motschulsky) feeds on living, healthy host trees and harbors a diverse gut microbial community. We investigated gut microbial contributions to larval nitrogen requirements through nitrogen fixing and recycling (urea hydrolysis) processes, using a combination of molecular, biochemical, and stable isotope approaches. Genes and transcripts of conserved regions of the urease operon (ureC) and nitrogen fixing (nif) regulon (nifH) were detected in A. glabripennis eggs and larvae from naturally infested logs and from larvae reared on artificial diet. Significant nitrogen fixation and recycling were documented in larvae using 15N2 gas and 15N-urea, respectively. Subsequent 15N-routing of incorporated recycled nitrogen into larval essential and nonessential amino acids was shown for 15N-urea diet-fed larvae. Results from this study show significant gut microbial contributions to this insect's metabolic nitrogen utilization through nitrogenous waste product recycling and nitrogen fixation.

Original languageEnglish (US)
Pages (from-to)903-912
Number of pages10
JournalEnvironmental Entomology
Volume43
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Nitrogen
digestive system
microorganisms
Larva
nitrogen
intestinal microorganisms
Anoplophora glabripennis
urea
recycling
larva
Urea
Nitrogen Fixation
larvae
Recycling
nitrogen fixation
Diet
Insects
Equipment Reuse
insect
diet

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Medicine(all)

Cite this

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title = "Gut microbes contribute to nitrogen provisioning in a wood-feeding cerambycid",
abstract = "Xylophagous insects often thrive on nutritionally suboptimal diets through symbiotic associations with microbes that supplement their nutritional requirements, particularly nitrogen. The wood-feeding cerambycid Anoplophora glabripennis (Motschulsky) feeds on living, healthy host trees and harbors a diverse gut microbial community. We investigated gut microbial contributions to larval nitrogen requirements through nitrogen fixing and recycling (urea hydrolysis) processes, using a combination of molecular, biochemical, and stable isotope approaches. Genes and transcripts of conserved regions of the urease operon (ureC) and nitrogen fixing (nif) regulon (nifH) were detected in A. glabripennis eggs and larvae from naturally infested logs and from larvae reared on artificial diet. Significant nitrogen fixation and recycling were documented in larvae using 15N2 gas and 15N-urea, respectively. Subsequent 15N-routing of incorporated recycled nitrogen into larval essential and nonessential amino acids was shown for 15N-urea diet-fed larvae. Results from this study show significant gut microbial contributions to this insect's metabolic nitrogen utilization through nitrogenous waste product recycling and nitrogen fixation.",
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Gut microbes contribute to nitrogen provisioning in a wood-feeding cerambycid. / Ayayee, Paul; Rosa, Cristina; Ferry, James Gregory; Felton, Gary; Saunders, Mike; Hoover, Kelli.

In: Environmental Entomology, Vol. 43, No. 4, 2014, p. 903-912.

Research output: Contribution to journalArticle

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