Gut microbes contribute to nitrogen provisioning in a wood-feeding cerambycid

Paul Ayayee, Cristina Rosa, James Gregory Ferry, Gary Felton, Michael Craig Saunders, Kelli Hoover

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Xylophagous insects often thrive on nutritionally suboptimal diets through symbiotic associations with microbes that supplement their nutritional requirements, particularly nitrogen. The wood-feeding cerambycid Anoplophora glabripennis (Motschulsky) feeds on living, healthy host trees and harbors a diverse gut microbial community. We investigated gut microbial contributions to larval nitrogen requirements through nitrogen fixing and recycling (urea hydrolysis) processes, using a combination of molecular, biochemical, and stable isotope approaches. Genes and transcripts of conserved regions of the urease operon (ureC) and nitrogen fixing (nif) regulon (nifH) were detected in A. glabripennis eggs and larvae from naturally infested logs and from larvae reared on artificial diet. Significant nitrogen fixation and recycling were documented in larvae using 15N2 gas and 15N-urea, respectively. Subsequent 15N-routing of incorporated recycled nitrogen into larval essential and nonessential amino acids was shown for 15N-urea diet-fed larvae. Results from this study show significant gut microbial contributions to this insect's metabolic nitrogen utilization through nitrogenous waste product recycling and nitrogen fixation.

Original languageEnglish (US)
Pages (from-to)903-912
Number of pages10
JournalEnvironmental Entomology
Volume43
Issue number4
DOIs
StatePublished - Jan 1 2014

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All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Insect Science

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