Abstract
The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the present study, 36 diverse indole derivatives were investigated with the aim of identifying novel compounds that inhibit persisters and biofilm formation by Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. 5-Iodoindole and other halogenated indoles, 4-fluoroindole, 7-chloroindole, and 7-bromoindole, eradicated persister formation by E. coli and S. aureus, and 5-iodoindole most potently inhibited biofilm formation by the two bacteria. Unlike other antibiotics, 5-iodoindole did not induce persister cell formation, and 5-iodoindole inhibited the production of the immune-evasive carotenoid staphyloxanthin in S. aureus; hence, 5-iodoindole diminished the production of virulence factors in this strain. These results demonstrate halogenated indoles are potentially useful for controlling bacterial antibiotic resistance.
| Original language | English (US) |
|---|---|
| Article number | 123 |
| Journal | AMB Express |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1 2016 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Applied Microbiology and Biotechnology
- Orthopedics and Sports Medicine
- Physiology (medical)
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Halogenated indoles eradicate bacterial persister cells and biofilms. / Lee, Jin Hyung; Kim, Yong Guy; Gwon, Giyeon; Wood, Thomas Keith; Lee, Jintae.
In: AMB Express, Vol. 6, No. 1, 123, 01.12.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Halogenated indoles eradicate bacterial persister cells and biofilms
AU - Lee, Jin Hyung
AU - Kim, Yong Guy
AU - Gwon, Giyeon
AU - Wood, Thomas Keith
AU - Lee, Jintae
PY - 2016/12/1
Y1 - 2016/12/1
N2 - The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the present study, 36 diverse indole derivatives were investigated with the aim of identifying novel compounds that inhibit persisters and biofilm formation by Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. 5-Iodoindole and other halogenated indoles, 4-fluoroindole, 7-chloroindole, and 7-bromoindole, eradicated persister formation by E. coli and S. aureus, and 5-iodoindole most potently inhibited biofilm formation by the two bacteria. Unlike other antibiotics, 5-iodoindole did not induce persister cell formation, and 5-iodoindole inhibited the production of the immune-evasive carotenoid staphyloxanthin in S. aureus; hence, 5-iodoindole diminished the production of virulence factors in this strain. These results demonstrate halogenated indoles are potentially useful for controlling bacterial antibiotic resistance.
AB - The emergence of antibiotic resistance has necessitated new therapeutic approaches to combat recalcitrant bacterial infections. Persister cells, often found in biofilms, are metabolically dormant, and thus, are highly tolerant to all traditional antibiotics and represent a major drug resistance mechanism. In the present study, 36 diverse indole derivatives were investigated with the aim of identifying novel compounds that inhibit persisters and biofilm formation by Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. 5-Iodoindole and other halogenated indoles, 4-fluoroindole, 7-chloroindole, and 7-bromoindole, eradicated persister formation by E. coli and S. aureus, and 5-iodoindole most potently inhibited biofilm formation by the two bacteria. Unlike other antibiotics, 5-iodoindole did not induce persister cell formation, and 5-iodoindole inhibited the production of the immune-evasive carotenoid staphyloxanthin in S. aureus; hence, 5-iodoindole diminished the production of virulence factors in this strain. These results demonstrate halogenated indoles are potentially useful for controlling bacterial antibiotic resistance.
UR - http://www.scopus.com/inward/record.url?scp=85002487986&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85002487986&partnerID=8YFLogxK
U2 - 10.1186/s13568-016-0297-6
DO - 10.1186/s13568-016-0297-6
M3 - Article
VL - 6
JO - AMB Express
JF - AMB Express
SN - 2191-0855
IS - 1
M1 - 123
ER -