Of the seven non-ionic detergents tested, Span 40®, Tween 20® and Triton X-45® were suitable for extraction of haptoglobin (Hp) from fatty tissues. The extracted samples were subjected to electrophoresis and subsequently stained with o-tolidine. Enzyme-immunoassay and Western blotting (electrophoretic) techniques were used successfully to determine the Hp phenotypes from minute quantities of tissue samples after extractions with non-ionic detergents.
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine