Harnessing a physiologic mechanism for siRNA delivery with mimetic lipoprotein particles

Tomoko Nakayama, James S. Butler, Alfica Sehgal, Mariano Severgnini, Tim Racie, Jennifer Sharman, Feng Ding, Svetlana Shulga Morskaya, Joshua Brodsky, Lubomir Tchangov, Verbena Kosovrasti, Mike Meys, Lubomir Nechev, Gang Wang, Chang Geng Peng, Yupang Fang, Martin Maier, Kallanthottathil G. Rajeev, Robert Li, Julia HettingerScott Barros, Valerie Clausen, Xuemei Zhang, Qianfan Wang, Renta Hutabarat, Nikolay V. Dokholyan, Christian Wolfrum, Muthiah Manoharan, Victor Kotelianski, Markus Stoffel, Dinah W.Y. Sah

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Therapeutics based on RNA interference (RNAi) have emerged as a potential new class of drugs for treating human disease by silencing the target messenger RNA (mRNA), thereby reducing levels of the corresponding pathogenic protein. The major challenge for RNAi therapeutics is the development of safe delivery vehicles for small interfering RNAs (siRNAs). We previously showed that cholesterol-conjugated siRNAs (chol-siRNA) associate with plasma lipoprotein particles and distribute primarily to the liver after systemic administration to mice. We further demonstrated enhancement of silencing by administration of chol-siRNA pre-associated with isolated high-density lipoprotein (HDL) or low-density lipoprotein (LDL). In this study, we investigated mimetic lipoprotein particle prepared from recombinant apolipoprotein A1 (apoA) and apolipoprotein E3 (apoE) as a delivery vehicle for chol-siRNAs. We show that apoE-containing particle (E-lip) is highly effective in functional delivery of chol-siRNA to mouse liver. E-lip delivery was found to be considerably more potent than apoA-containing particle (A-lip). Furthermore, E-lip-mediated delivery was not significantly affected by high endogenous levels of plasma LDL. These results demonstrate that E-lip has substantial potential as delivery vehicles for lipophilic conjugates of siRNAs.

Original languageEnglish (US)
Pages (from-to)1582-1589
Number of pages8
JournalMolecular Therapy
Volume20
Issue number8
DOIs
StatePublished - Aug 2012

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

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