@article{783a0df35322478dace53c7cb343d163,
title = "Hedgehog-Activated Fat4 and PCP Pathways Mediate Mesenchymal Cell Clustering and Villus Formation in Gut Development",
abstract = "Rao-Bhatia et al. investigate the signaling mechanisms of cell behavior during mammalian villus morphogenesis. They demonstrate that Hh signaling in the intestinal mesenchyme directly activates planar cell polarity genes, thereby regulating the stromal cell clustering behavior required for epithelial remodeling in villus formation.",
author = "Abilasha Rao-Bhatia and Min Zhu and Yin, {Wen Chi} and Sabrina Coquenlorge and Xiaoyun Zhang and Janghee Woo and Yu Sun and Dean, {Charlotte H.} and Aimin Liu and Hui, {Chi chung} and Shivdasani, {Ramesh A.} and Helen McNeill and Sevan Hopyan and Kim, {Tae Hee}",
note = "Funding Information: This work was supported by SickKids Foundation start-up, the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery # RGPIN-2016-06093 grant, and the March of Dimes Basil O{\textquoteright}Connor Starter Scholar Research Award (T.-H.K.); the SickKids Restracomp and Ontario Graduate Scholarships (A.R.-B.); the grants U.S. NSF IOS-1257540 and U.S. NIH HD083625 (A.L.); NSERC Discovery and the Canadian Institutes of Health Research grants (C.-C.H.); NIH grant R01DK081113 (R.A.S.); and Canadian Institutes of Health Research (CIHR) foundation grant (H.M., a BJC investigator and a Larry J. and Carol A. Shapiro Professor at Washington University School of Medicine). We thank Dr. Warren Zimmer for Bapx1 Cre mice, Dr. Philippe Gros for VANGL2 antibodies, and Edward Espinoza for helpful discussion. Funding Information: This work was supported by SickKids Foundation start-up, the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery #RGPIN-2016-06093 grant, and the March of Dimes Basil O'Connor Starter Scholar Research Award (T.-H.K.); the SickKids Restracomp and Ontario Graduate Scholarships (A.R.-B.); the grants U.S. NSF IOS-1257540 and U.S. NIH HD083625 (A.L.); NSERC Discovery and the Canadian Institutes of Health Research grants (C.-C.H.); NIH grant R01DK081113 (R.A.S.); and Canadian Institutes of Health Research (CIHR) foundation grant (H.M. a BJC investigator and a Larry J. and Carol A. Shapiro Professor at Washington University School of Medicine). We thank Dr. Warren Zimmer for Bapx1Cre mice, Dr. Philippe Gros for VANGL2 antibodies, and Edward Espinoza for helpful discussion. A.R.-B. designed and performed the experiments, analyzed the data, and wrote the manuscript; M.Z. performed and analyzed the live-imaging data; W.-C.Y. performed the ChIP-seq and analyzed the data; S.C. performed the RNA-seq and ChIP-seq; X.Z. helped with the ChIP-seq validation studies; J.W. helped with the cell culture studies; Y.S. supervised the imaging analysis; C.H.D. provided the PCP mutant embryos; A.L. provided the PCP mutant embryos; C.-C.H. provided the Hh mutant mice and supervised the bioinformatic analyses of ChIP-seq and RNA-seq; R.A.S. supervised the initiation of this project; H.M. provided the Fat4, Dchs1, and Vangl2 mutant mice and helpful suggestions; S.H. supervised the imaging analysis; and T.-H.K. conceived and supervised the study, analyzed the data, and wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = mar,
day = "9",
doi = "10.1016/j.devcel.2020.02.003",
language = "English (US)",
volume = "52",
pages = "647--658.e6",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",
}