Hedgehog signalling in the mouse requires intraflagellar transport proteins

Danwei Huangfu, Aimin Liu, Andrew S. Rakeman, Noel S. Murcia, Lee Niswander, Kathryn V. Anderson

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891 Scopus citations

Abstract

Intraflagellar transport (IFT) proteins were first identified as essential factors for the growth and maintenance of flagella in the single-celled alga Chlamydomonas reinhardtii. In a screen for embryonic patterning mutations induced by ethylnitrosourea, here we identify two mouse mutants, wimple (wim) and flexo (fxo), that lack ventral neural cell types and show other phenotypes characteristic of defects in Sonic hedgehog signalling. Both mutations disrupt IFT proteins: the wim mutation is an allele of the previously uncharacterized mouse homologue of IFT172; and fxo is a new hypomorphic allele of polaris, the mouse homologue of IFT88. Genetic analysis shows that Wim, Polaris and the IFT motor protein Kif3a are required for Hedgehog signalling at a step downstream of Patched1 (the Hedgehog receptor) and upstream of direct targets of Hedgehog signalling. Our data show that IFT machinery has an essential and vertebrate-specific role in Hedgehog signal transduction.

Original languageEnglish (US)
Pages (from-to)83-87
Number of pages5
JournalNature
Volume426
Issue number6962
DOIs
StatePublished - Nov 6 2003

All Science Journal Classification (ASJC) codes

  • General

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    Huangfu, D., Liu, A., Rakeman, A. S., Murcia, N. S., Niswander, L., & Anderson, K. V. (2003). Hedgehog signalling in the mouse requires intraflagellar transport proteins. Nature, 426(6962), 83-87. https://doi.org/10.1038/nature02061