Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice

L. A. Dieleman, A. Arends, S. L. Tonkonogy, M. S. Goerres, David Craft, W. Grenther, R. K. Sellon, E. Balish, R. B. Sartor

Research output: Contribution to journalArticle

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Abstract

Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10(-/-) mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10(-/-) mice. Germfree IL-10(-/-) mice on either a mixed (C57BL/6 x 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10(-/-) mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus. After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10(-/-) mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus. We conclude that H. hepaticus does not induce or potentiate disease in our IL-10(-/-) mice and therefore is not required to induce colitis in genetically susceptible hosts.

Original languageEnglish (US)
Pages (from-to)5107-5113
Number of pages7
JournalInfection and Immunity
Volume68
Issue number9
DOIs
StatePublished - Sep 14 2000

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Helicobacter hepaticus
Colitis
Interleukin-10
Specific Pathogen-Free Organisms
Interleukin-12
Weight Loss
T-Lymphocytes
Mortality
Enema
Knockout Mice
Hepatitis
Hepatocellular Carcinoma
Histology
Colon
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Dieleman, L. A., Arends, A., Tonkonogy, S. L., Goerres, M. S., Craft, D., Grenther, W., ... Sartor, R. B. (2000). Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice. Infection and Immunity, 68(9), 5107-5113. https://doi.org/10.1128/IAI.68.9.5107-5113.2000
Dieleman, L. A. ; Arends, A. ; Tonkonogy, S. L. ; Goerres, M. S. ; Craft, David ; Grenther, W. ; Sellon, R. K. ; Balish, E. ; Sartor, R. B. / Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice. In: Infection and Immunity. 2000 ; Vol. 68, No. 9. pp. 5107-5113.
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Dieleman, LA, Arends, A, Tonkonogy, SL, Goerres, MS, Craft, D, Grenther, W, Sellon, RK, Balish, E & Sartor, RB 2000, 'Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice', Infection and Immunity, vol. 68, no. 9, pp. 5107-5113. https://doi.org/10.1128/IAI.68.9.5107-5113.2000

Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice. / Dieleman, L. A.; Arends, A.; Tonkonogy, S. L.; Goerres, M. S.; Craft, David; Grenther, W.; Sellon, R. K.; Balish, E.; Sartor, R. B.

In: Infection and Immunity, Vol. 68, No. 9, 14.09.2000, p. 5107-5113.

Research output: Contribution to journalArticle

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T1 - Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice

AU - Dieleman, L. A.

AU - Arends, A.

AU - Tonkonogy, S. L.

AU - Goerres, M. S.

AU - Craft, David

AU - Grenther, W.

AU - Sellon, R. K.

AU - Balish, E.

AU - Sartor, R. B.

PY - 2000/9/14

Y1 - 2000/9/14

N2 - Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10(-/-) mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10(-/-) mice. Germfree IL-10(-/-) mice on either a mixed (C57BL/6 x 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10(-/-) mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus. After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10(-/-) mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus. We conclude that H. hepaticus does not induce or potentiate disease in our IL-10(-/-) mice and therefore is not required to induce colitis in genetically susceptible hosts.

AB - Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10(-/-) mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10(-/-) mice. Germfree IL-10(-/-) mice on either a mixed (C57BL/6 x 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10(-/-) mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus. After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10(-/-) mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus. We conclude that H. hepaticus does not induce or potentiate disease in our IL-10(-/-) mice and therefore is not required to induce colitis in genetically susceptible hosts.

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