Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice

L. A. Dieleman, A. Arends, S. L. Tonkonogy, M. S. Goerres, D. W. Craft, W. Grenther, R. K. Sellon, E. Balish, R. B. Sartor

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Helicobacter hepaticus has been reported to induce colitis, hepatitis, and hepatocellular carcinoma in several different murine models. The aim of this study was to determine if H. hepaticus will cause colitis in monoassociated mice lacking the interleukin-10 gene (IL-10(-/-) mice) and potentiate colitis in specific-pathogen-free (SPF) IL-10(-/-) mice. Germfree IL-10(-/-) mice on either a mixed (C57BL/6 x 129/Ola) or inbred (129/SvEv) genetic background were monoassociated with H. hepaticus ATCC 51448 by oral feeding and rectal enemas. In a second experiment, germfree IL-10(-/-) mice were colonized with stool from SPF mice that harbored or did not harbor endogenous H. hepaticus. After 7 to 9 weeks of colonization, weight loss and mortality were assessed, the colon was isolated for histology and IL-12 secretion, and mesenteric lymph node cells were assessed for T-cell activation markers. It was found that IL-10(-/-) mice monoassociated with H. hepaticus for up to 16 weeks showed almost no histologic colitis or increased IL-12 production. SPF IL-10-knockout mice had no significant difference in weight loss, mortality rate, histologic scores, colonic IL-12 secretion, or T-cell activation with or without H. hepaticus. We conclude that H. hepaticus does not induce or potentiate disease in our IL-10(-/-) mice and therefore is not required to induce colitis in genetically susceptible hosts.

Original languageEnglish (US)
Pages (from-to)5107-5113
Number of pages7
JournalInfection and Immunity
Volume68
Issue number9
DOIs
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice'. Together they form a unique fingerprint.

  • Cite this

    Dieleman, L. A., Arends, A., Tonkonogy, S. L., Goerres, M. S., Craft, D. W., Grenther, W., Sellon, R. K., Balish, E., & Sartor, R. B. (2000). Helicobacter hepaticus does not induce or potentiate colitis in interleukin-10-deficient mice. Infection and Immunity, 68(9), 5107-5113. https://doi.org/10.1128/IAI.68.9.5107-5113.2000