Hemophilus influenzae meningitis in the rat: Behavioral, electrophysiological, and biochemical consequences

Richard J. Konkol, Louisa Chapman, George R. Breese, Albert M. Collier, Clinton Kilts, Charles Finley, Richard R. Vogel, Richard Mailman, Elizabeth G. Bendeich

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Hemophilus influenzae is the most common cause of bacterial meningitis in children, and a high percentage of survivors are at risk for long‐term sequelae. To explore the mechanisms responsible for these sequelae, a neonatal rat model was used to define the behavioral, electrophysiological, and biochemical changes following meningitis. Three days after inoculation of 6‐day‐old rats with a minimum of 1 × 107 colony‐forming units of a virulent Hemophilus influenzae, type b, cerebrospinal fluid and blood were cultured to confirm the presence of meningitis and bacteremia, respectively. At this time, forebrain norepinephrine and dopamine levels were significantly elevated in meningitic rats when standardized on a wet‐weight basis. No changes in brain serotonin or heart norepinephrine levels could be found in the 9‐day‐old rats. No residual changes were found in steady‐state concentrations of norepinephrine or dopamine in surviving adult rats. However, survivors that had had meningitis as neonates showed significant impairment in active and passive avoidance learning tasks and demonstrated a significantly higher level of activity during a habituation period in circular photocell activity cages. No change in the flinch‐jump threshold was detected. Brainstem auditory evoked potentials showed delays of various waves in 3 of 10 Hemophilus influenzae type b–treated adult rats tested. These rats also exhibited markedly augmented locomotory responses to d‐amphetamine (1 mg/kg), suggesting a long‐lasting perturbation of central monoamine neuronal transmission.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalAnnals of Neurology
Volume21
Issue number4
DOIs
StatePublished - Jan 1 1987

Fingerprint

Haemophilus Meningitis
Haemophilus influenzae
Meningitis
Norepinephrine
Dopamine
Avoidance Learning
Haemophilus influenzae type b
Bacterial Meningitides
Brain Stem Auditory Evoked Potentials
Prosencephalon
Bacteremia
Cerebrospinal Fluid
Serotonin
Newborn Infant
Brain

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Konkol, R. J., Chapman, L., Breese, G. R., Collier, A. M., Kilts, C., Finley, C., ... Bendeich, E. G. (1987). Hemophilus influenzae meningitis in the rat: Behavioral, electrophysiological, and biochemical consequences. Annals of Neurology, 21(4), 353-360. https://doi.org/10.1002/ana.410210407
Konkol, Richard J. ; Chapman, Louisa ; Breese, George R. ; Collier, Albert M. ; Kilts, Clinton ; Finley, Charles ; Vogel, Richard R. ; Mailman, Richard ; Bendeich, Elizabeth G. / Hemophilus influenzae meningitis in the rat : Behavioral, electrophysiological, and biochemical consequences. In: Annals of Neurology. 1987 ; Vol. 21, No. 4. pp. 353-360.
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Konkol, RJ, Chapman, L, Breese, GR, Collier, AM, Kilts, C, Finley, C, Vogel, RR, Mailman, R & Bendeich, EG 1987, 'Hemophilus influenzae meningitis in the rat: Behavioral, electrophysiological, and biochemical consequences', Annals of Neurology, vol. 21, no. 4, pp. 353-360. https://doi.org/10.1002/ana.410210407

Hemophilus influenzae meningitis in the rat : Behavioral, electrophysiological, and biochemical consequences. / Konkol, Richard J.; Chapman, Louisa; Breese, George R.; Collier, Albert M.; Kilts, Clinton; Finley, Charles; Vogel, Richard R.; Mailman, Richard; Bendeich, Elizabeth G.

In: Annals of Neurology, Vol. 21, No. 4, 01.01.1987, p. 353-360.

Research output: Contribution to journalArticle

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T2 - Behavioral, electrophysiological, and biochemical consequences

AU - Konkol, Richard J.

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AU - Bendeich, Elizabeth G.

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